Chapter

Handbook Of Biological Confocal Microscopy

pp 43-58

Special Optical Elements

  • Jens RietdorfAffiliated withEuropean Molecular Biology Laboratory (EMBL), Advanced Light Microscopy Facility and Light Microscopy Group
  • , Ernst H. K. StelzerAffiliated withEuropean Molecular Biology Laboratory (EMBL), Advanced Light Microscopy Facility and Light Microscopy Group

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Abstract

The amazing array of microscopic instrumentation for generating three-dimensional images of biological specimens that are described elsewhere in this volume rely on a relatively small number of optical and mechanical components. Although most biologists have a general idea of their function and how to operate them, some mysteries remain.