Handbook Of Biological Confocal Microscopy

pp 516-534

Fluorescence Lifetime Imaging in Scanning Microscopy

  • H. C. GerritsenAffiliated withUtrecht University
  • , A. DraaijerAffiliated withTNO-Voeding
  • , D. J. van den HeuvelAffiliated withUtrecht University
  • , A. V. AgronskaiaAffiliated withUtrecht University

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It was not until the 1930s that measurement of the fluorescence lifetime (τ f ) and the confirmation of theory on this phenomenon became possible (Pringsheim, 1961). Before that time only phosphorescence lifetimes had been measured, evidently because this phenomenon is one or more orders of magnitude slower.