Chapter

Handbook Of Biological Confocal Microscopy

pp 404-413

Aberrations in Confocal and Multi-Photon Fluorescence Microscopy Induced by Refractive Index Mismatch

  • Alexander EgnerAffiliated withDepartment of NanoBiophotonics, Max Planck-Institute for Biophysical Chemistry
  • , Stefan W. HellAffiliated withDepartment of NanoBiophotonics, Max Planck-Institute for Biophysical Chemistry

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Modern optical microscopes are so good that many scientists forget that these instruments only provide their optimal performance if they are used under certain operating conditions. Typical users may be unaware of the very existence of such limitations either because they may unwittingly work within the limits or because they fail to recognize their effects. It is probably also correct to assume that the manufacturer does not intend to discourage purchase by emphasizing the pitfalls that unavoidably arise from the physics of imaging.