Chapter

Microarray Technology and Cancer Gene Profiling

Volume 593 of the series Advances in Experimental Medicine and Biology pp 66-73

Complementary Techniques

Validation of Gene Expression Data by Quantitative Real Time PCR
  • Maurizio ProvenzanoAffiliated withDepartment of Oncological and Surgical Sciences, University of Padova
  • , Simone MocellinAffiliated withDepartment of Oncological and Surgical Sciences, University of Padova Email author 

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Abstract

Microarray technology can be considered the most powerful tool for screening gene expression profiles of biological samples. After data mining, results need to be validated with highly reliable biotechniques allowing for precise quantitation of transcriptional abundance of identified genes. Quantitative real time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstrumentation for gene level measurement. Currently, qrt-PCR is considered by most experts the most appropriate method to confirm or confute microarray-generated data. The knowledge of the biochemical principles underlying qrt-PCR as well as some related technical issues must be beard in mind when using this biotechnology.