Reconstructing the Optical Thickness from Hoffman Modulation Contrast Images
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- Olsen N.H., Sporring J., Nielsen M., Hnida C., Ziebe S. (2003) Reconstructing the Optical Thickness from Hoffman Modulation Contrast Images. In: Bigun J., Gustavsson T. (eds) Image Analysis. SCIA 2003. Lecture Notes in Computer Science, vol 2749. Springer, Berlin, Heidelberg
Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness.
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