Chapter

Image Analysis

Volume 2749 of the series Lecture Notes in Computer Science pp 526-533

Date:

Reconstructing the Optical Thickness from Hoffman Modulation Contrast Images

  • Niels Holm OlsenAffiliated with3D-Lab, School of Dentistry, University of CopenhagenIT University of CopenhagenIH-Medical, Image House A/S
  • , Jon SporringAffiliated with3D-Lab, School of Dentistry, University of Copenhagen
  • , Mads NielsenAffiliated withIT University of Copenhagen
  • , Christina HnidaAffiliated withFertility Clinic, University HospitalIH-Medical, Image House A/S
  • , Søren ZiebeAffiliated withFertility Clinic, University Hospital

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Abstract

Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness.