Reconstructing the Optical Thickness from Hoffman Modulation Contrast Images

  • Niels Holm Olsen
  • Jon Sporring
  • Mads Nielsen
  • Christina Hnida
  • Søren Ziebe
Conference paper

DOI: 10.1007/3-540-45103-X_70

Part of the Lecture Notes in Computer Science book series (LNCS, volume 2749)
Cite this paper as:
Olsen N.H., Sporring J., Nielsen M., Hnida C., Ziebe S. (2003) Reconstructing the Optical Thickness from Hoffman Modulation Contrast Images. In: Bigun J., Gustavsson T. (eds) Image Analysis. SCIA 2003. Lecture Notes in Computer Science, vol 2749. Springer, Berlin, Heidelberg

Abstract

Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • Niels Holm Olsen
    • 1
    • 2
    • 4
  • Jon Sporring
    • 1
  • Mads Nielsen
    • 2
  • Christina Hnida
    • 3
    • 4
  • Søren Ziebe
    • 3
  1. 1.3D-Lab, School of DentistryUniversity of CopenhagenCopenhagenDenmark
  2. 2.IT University of CopenhagenCopenhagenDenmark
  3. 3.Fertility ClinicUniversity HospitalCopenhagenDenmark
  4. 4.IH-Medical, Image House A/SCopenhagenDenmark

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