Chapter

RNA Towards Medicine

Volume 173 of the series Handbook of Experimental Pharmacology pp 97-104

High-Throughput RNA Interference in Functional Genomics

  • M. JanitzAffiliated withDepartment Vertebrate Genomics, Max Planck Institute for Molecular Genetics
  • , D. VanheckeAffiliated withDepartment Vertebrate Genomics, Max Planck Institute for Molecular Genetics
  • , H. LehrachAffiliated withDepartment Vertebrate Genomics, Max Planck Institute for Molecular Genetics

* Final gross prices may vary according to local VAT.

Get Access

Abstract

RNA interference (RNAi) refers to post-transcriptional silencing of gene expression as a result of the introduction of double-stranded RNA into cells. The application of RNAi in experimental systems has significantly accelerated elucidation of gene functions. In order to facilitate large-scale functional genomics studies using RNAi, several high-throughput approaches have been developed based on microarray or microwell assays. The recent establishment of large libraries of RNAi reagents combined with a variety of detection assays has further improved the performance of functional genome-wide screens in mammalian cells.

Keywords

RNA interference Microarray Reverse transfection Cell array