Chapter

Biopolymers/Non-Exclusion HPLC

Volume 79 of the series Advances in Polymer Science pp 1-63

Date:

Protein adsorption and materials biocompatibility: A tutorial review and suggested hypotheses

  • J. D. AndradeAffiliated withCollege of Engineering, University of Utah
  • , V. HladyAffiliated withCollege of Engineering, University of Utah

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Abstract

A comprehensive review of protein adsorption at solid-liquid interfaces is presented, including a brief review of protein structure and the principles of protein adsorption. Adsorption-based biocompatibility hypotheses and correlations are discussed, including surface charge, interface energetics, passivation, protein-resistant surfaces, and the role of adsorbed immunoglobulins and complement. New methods for the study of protein adsorption are discussed, including total internal reflection techniques (absorbance, fluorescence, and Raman) and ellipsometry. Qualitative “rules of thumb” of protein adsorption are also presented.