Chapter

Activity-Based Protein Profiling

Volume 324 of the series Topics in Current Chemistry pp 137-162

Date:

Functional Analysis of Protein Targets by Metabolomic Approaches

  • Yun-Gon KimAffiliated withDepartment of Chemistry and Chemical Biology, Harvard University
  • , Alan SaghatelianAffiliated withDepartment of Chemistry and Chemical Biology, Harvard University Email author 

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Abstract

Proteomics methods, such as activity-based protein profiling, can be used to connect proteins to biology and disease. Some proteins found through unbiased methods are not well characterized, which makes it difficult to ascertain the role of these proteins. Metabolomics approaches are useful in characterizing proteins that regulate or bind metabolites. Here, we provide examples of the development and use of metabolomics approaches to elucidate protein–metabolite interactions.

Keywords

Enzymes Liquid chromatography-mass spectrometry (LC-MS) Metabolite binding proteins Metabolomics