Chapter

Prion Proteins

Volume 305 of the series Topics in Current Chemistry pp 121-133

Date:

Prion Seeded Conversion and Amplification Assays

  • Christina D. OrrúAffiliated withLaboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health
  • , Byron CaugheyAffiliated withLaboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health Email author 

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Abstract

The conversion of the normal prion protein (PrPC) into its misfolded, aggregation-prone and infectious (prion) isoform is central to the progression of transmissible spongiform encephalopathies (TSEs) or prion diseases. Since the initial development of a cell free PrP conversion reaction, striking progress has been made in the development of much more continuous prion-induced conversion and amplification reactions. These studies have provided major insights into the molecular underpinnings of prion propagation and enabled the development of ultra-sensitive tests for prions and prion disease diagnosis. This chapter will provide an overview of such reactions and the practical and fundamental consequences of their development.

Keywords

Prion composition PMCA QuIC ASA Transmissible Spongiform Ecephalopathies