Chapter

Disposable Bioreactors

Volume 115 of the series Advances in Biochemical Engineering / Biotechnology pp 55-87

Date:

Bag Bioreactor Based on Wave-Induced Motion: Characteristics and Applications

  • Regine EiblAffiliated withInstitute of Biotechnology, Zurich University of Applied Sciences, School of Life Sciences and Facility Management Email author 
  • , Sören WernerAffiliated withInstitute of Biotechnology, Zurich University of Applied Sciences, School of Life Sciences and Facility Management
  • , Dieter EiblAffiliated withInstitute of Biotechnology, Zurich University of Applied Sciences, School of Life Sciences and Facility Management

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Abstract

Today wave-mixed bag bioreactors are common devices in modern biotechnological processes where simple, safe and flexible production has top priority. Numerous studies that have been published on ex vivo generation of cells, viruses and therapeutic agents during the last 10 years have confirmed their suitability and even superiority to stirred bioreactors made from glass or stainless steel for animal as well as plant cell cultivations. In these studies the wave-mixed bag bioreactors enabled middle to high cell density and adequate productivity in laboratory and pilot scale. This mainly results from low-shear conditions and highly efficient oxygen transfer for cell cultures, as demonstrated for the widely used BioWave®.Starting with an overview of wave-mixed bag bioreactors and their common operation strategies, this chapter delineates engineering aspects of BioWave®, which like Wave Reactor™ and BIOSTAT®CultiBag RM originates from the prototype of a wave-mixed bag bioreactor introduced in 1998. Subsequently, the second part of the chapter focuses on reported BioWave® applications. Conditions and results from cultivations with animal cells, plant cells, microbial cells and nematodes are presented and discussed.

Keywords

Animal cells Biological insecticides Biotransformation Immunomodulator Plant cells Seed inoculum Wave-mixed bag bioreactor