Comparison of secondary plant metabolite production in cell suspension, callus culture and temporary immersion system

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Abstract

Cell and organ cultures of Lavandula officinalis, Hypericum perforatum, Cymbopogon citratus and Fabiana imbricata were established for the production of secondary metabolites in vitro. Shoot multiplication was performed by conventional micropropagation on agar-solidified medium as well as in temporary immersion systems (TIS), the latter resulted in higher multiplication rates compared to the culture in microcontainers for all plant species tested. The concentration of bioactive compounds was determined in different in vitro cell and organ cultures and was compared to field grown plants. For Lavandula the highest content of rosmarinic acid was found in cell cultures, for the other three species in field grown plants. Concentrations of bioactive compounds were always higher in plant material grown in TIS compared to cell suspension and callus cultures.