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Cell and Organ Printing pp 95–113Cite as

High-Throughput Biological Laser Printing: Droplet Ejection Mechanism, Integration of a Dedicated Workstation, and Bioprinting of Cells and Biomaterials

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Abstract

High-Throughput Biological Laser Printing (HT BioLP) requires taking into account spatio-temporal proximity of laser pulses (that means pulse-to-pulse distance and laser pulse frequency). The droplet ejection mechanism is indeed governed by vapor bubble dynamics (bubble growth and collapsing) and it is thus related to both the condition of laser irradiation and the rheological properties of the liquid film (viscosity, surface tension). We present a rapid prototyping workstation which has been designed for HT BioLP applications. It is equipped with an infra-red pulsed laser (pulse duration = 30 ns, wavelength = 1,064 nm, f = 1–100 kHz), galvanometric mirrors (scanning speed up to 2,000 mm/s), micrometric translation stages (x, y, z) and a dedicated software. Then, after describing experimental conditions leading to the high resolution printing (including cell density, laser parameters, etc.) of biological components, we present some typical multi-component and 3D printings achieved using this workstation. Finally, considering different criteria (speed, inoquity, etc.) the potentiality of HT BioLP is discussed as an alternative technology in Tissue Engineering applications.

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Acknowledgments

The authors would like to thank GIS ‘Advanced Materials in Aquitaine’ and Région Aquitaine for financial support. In addition, the authors would like to thank Reine Bareille and Murielle Rémy for their help in cell culture experiments.

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Authors and Affiliations

  1. INSERM, U577, Bordeaux, F-33076, France

    Fabien Guillemot, Bertrand Guillotin, Sylvain Catros, Agnès Souquet, Virginie Keriquel, Jean-Christophe Fricain & Joëlle Amedee

  2. University of Victor Segalen Bordeaux 2, Bordeaux, F-33076, France

    Fabien Guillemot, Bertrand Guillotin, Sylvain Catros, Agnès Souquet, Virginie Keriquel, Jean-Christophe Fricain & Joëlle Amedee

  3. Centre Lasers Intenses et Applications, UMR 5107 CEA V CNRS – Université Bordeaux 1, 33405, Cedex, France

    Candice Mezel & Ludovic Hallo

Authors
  1. Fabien Guillemot
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  2. Bertrand Guillotin
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  3. Sylvain Catros
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  4. Agnès Souquet
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  5. Candice Mezel
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  6. Virginie Keriquel
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  7. Ludovic Hallo
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  8. Jean-Christophe Fricain
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  9. Joëlle Amedee
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Corresponding author

Correspondence to Fabien Guillemot .

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Editors and Affiliations

  1. Div. Chemistry, Code 6113, Naval Research Laboratory (NRL), Overlook Ave. SW., 4555, Washington, 20375, District of Columbia, USA

    Bradley R. Ringeisen

  2. Chemical Dynamics and Diagnostics Branch, Code 6113, Naval Research Laboratory (NRL), Overlook Ave. SW., 4555, Washington, 20375, District of Columbia, USA

    Barry J. Spargo

  3. Div. Chemistry, Code 6113, Naval Research Laboratory (NRL), Overlook Ave. SW., 4555, Washington, 20375, District of Columbia, USA

    Peter K. Wu

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Guillemot, F. et al. (2010). High-Throughput Biological Laser Printing: Droplet Ejection Mechanism, Integration of a Dedicated Workstation, and Bioprinting of Cells and Biomaterials. In: Ringeisen, B., Spargo, B., Wu, P. (eds) Cell and Organ Printing. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9145-1_6

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