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Extracts from Fly Maggots and Fly Pupae as a “Wound Healer”

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Part of the book series: Parasitology Research Monographs ((Parasitology Res. Monogr.,volume 1))

Abstract

On one hand the population in good old Europe and North America is decreasing in number, yet on the other there is an enormous increase in age. This leads to an increase in typical diseases of the elderly such as diabetes, decubitus and/or “nonhealing wounds” of other origin as consequences of incorrect diet and/or being constantly bedridden. There are many approaches to clear the situation of those people with “nonhealing wounds”. However, although huge amounts of money are spent on different therapies thousands of amputations have to be carried out per year in most of the so-called industrialized countries. For example, in 2004 about 42,000 amputations were carried out in Germany as consequences of the typical diabetic-foot-syndrome that occurred among the 6-8 million people suffering in Germany from diabetes – worldwide there are more than 300 million humans involved in diabetic diseases. The chapter describes a new approach to heal “non-healing” wounds by use of a patented extract from larvae of the fly Lucilia sericata, which is finally lyophilyzed and thus can be stored for long before use as “wound cover”.

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References

  • Aisina RB, Mukhametova LI, Gulin DA, Levashov MY, Prisyazhnaya NV, Gershkovich KB, Varfolomeyev SD (2009) Inhibitory effect of angiostatins on activity of the plasminogen/plasminogen activator system. Biochemistry 74:1104–1113

    PubMed  CAS  Google Scholar 

  • Andriessen A, Polignano R, Abel M (2009) Monitoring the microcirculation to evaluate dressing performance in patients with venous leg ulcers. J Wound Care 18:145–150

    PubMed  CAS  Google Scholar 

  • Baer WS (1931) The treatment of chronic osteomyelitis with the maggot (larva of the blowfly). J Bone Jt Surg 13:438–475

    Google Scholar 

  • Beasley WD, Hirst G (2004) Making a meal of MRSA – the role of biosurgery in hospital-acquired infection. J Hosp Infect 56:6–9

    Article  PubMed  CAS  Google Scholar 

  • Bexfield A, Nigam Y, Thomas S, Ratcliffe NA (2004) Detection and partial characterisation of two antibacterial factors from the excretion/secretion of the medicinal maggot Lucilia sericata and their activity against methicillin-resistant Staphylococcus aureus (MRSA). Microbes Infect 6:1297–304

    Article  PubMed  CAS  Google Scholar 

  • Bexfield A, Bond AE, Roberts EC, Dudley E, Nigam Y, Thomas S, Newton S, Newton RP, Ratcliffe NA (2008) The antibacterial activity against MRSA strains and other bacteria of a <500 Da fraction from maggot excretions/secretions of Lucilia sericata (Diptera: Calliphoridae). Microbes Infect 10:325–333

    Article  PubMed  Google Scholar 

  • Bexfield A, Bond AE, Morgan C, Wagstaff J, Newton RP, Ratcliffe NA, Dudley E, Nigam Y (2010) Amino acid derivatives from Lucilia sericata excretions/secretions may contribute to the beneficial effects of maggot therapy via increased angiogenesis. Br J Dermatol 162:554–562

    Article  PubMed  CAS  Google Scholar 

  • Blair SE, Cokcetin NN, Harry EJ, Carter DA (2009) The unusual antibacterial activity of medical-grade Leptospermum honey: antibacterial spectrum, resistance and transcription analysis. Eur J Clin Microbiol Infect Dis 28:1199–1208

    Article  PubMed  CAS  Google Scholar 

  • Bonn D (2000) Maggot therapy: an alternative for wound infection. Lancet 356:1174

    Article  PubMed  CAS  Google Scholar 

  • Bowles VM, Carnegie PR, Sandeman RM (1988) Characterization of proteolytic and collagenolytic enzymes from the larvae of Lucilia cuprina, the sheep blowfly. Aust J Biol Res 41:269–278

    CAS  Google Scholar 

  • Bowles VM, Grey ST, Brandon MR (1992) Cellular immune response in the skin of sheep infected with larvae of Lucilia cuprina, the sheep blowfly. Vet Parasitol 44:151–162

    Article  PubMed  CAS  Google Scholar 

  • Bowles VM, Meeusen EN, Young AR, Andrews AE, Nash AD, Brandon MR (1996) Vaccination of sheep against larvae of the sheep blowfly (Lucilia cuprina). Vaccine 14:1347–1352

    Article  PubMed  CAS  Google Scholar 

  • Cassino R, Ricci E (2010) Effectiveness of tropical applications of amino acids to chronic wounds: a prospective observational study. J Wound Care 19:29–34

    PubMed  CAS  Google Scholar 

  • Casu RE, Jarmey JM, Elvin CM, Eisemann CH (2000) Isolation of a trypsin-like serine protease gene family from the sheep blowfly Lucilia cuprina. Insect Mol Biol 3:159–170

    Article  Google Scholar 

  • Cerovský V, Zdárek J, Fucík V, Monincová L, Voburka Z, Bém R (2010) Lucifensin, the long-sought antimicrobial factor of medicinal maggots of the blowfly Lucilia sericata. Cell Mol Life Sci 67:455–466

    Article  PubMed  Google Scholar 

  • Chambers L, Woodrow S, Brown AP, Harris PD, Phillips D, Hall M, Church JC, Pritchard DI (2003) Degradation of extracellular matrix components by defined proteinases from the greenbottle larva Lucilia sericata used for the clinical debridement of non-healing wounds. Br J Dermatol 148:14–23

    Article  PubMed  CAS  Google Scholar 

  • Chapman HA (1997) Plasminogen activators, integrins, and the coordinated regulation of cell adhesion and migration. Curr Opin Cell Biol 9:714–724

    Article  PubMed  CAS  Google Scholar 

  • Elkington RA, Humphries M, Commins M, Maugeri N, Tierney T, Mahony TJ (2009) Lucilia cuprina excretory-secretory protein inhibits the early phase of lymphocyte activation and subsequent proliferation. Parasite Immunol 31:750–765

    Article  PubMed  CAS  Google Scholar 

  • Evans P (2002) Larvae therapy and venous leg ulcers: reducing the “yuk factor”. J Wound Care 11:407–408

    PubMed  CAS  Google Scholar 

  • Fleischmann W, Grassberger M (2002) Maden-Therapie. Trias Verlag, Germany

    Google Scholar 

  • Förster M, Klimpel S, Mehlhorn H, Sievert K, Messler S, Pfeffer K (2007) Pilot study on synanthropic flies (e. g. Musca, Sarcophaga, Calliphora, Tannia. Lucilia, Stomoxys) as vectors of pathogenic microorganisms. Parasitol Res 101:243–246

    Article  PubMed  Google Scholar 

  • Foti C, Conserva A, Casullo C, Scrimieri V, Pepe ML, Quaranta D (2007) Contact dermatitis with clostridiopeptidase A contained in Noruxol ointment. Contact Dermatitia 56:361–362

    Article  Google Scholar 

  • George NM, Cutting KF (2007) Antibacterial Honey (MedihoneyTM): in-vitro activity against clinical isolates of MRSA, VRE, and other multiresistant gram negative organisms including Pseudomonas aeruginosa. Wound 19:231–236

    Google Scholar 

  • Gethin GT, Cowman S, Conroy RM (2008) The impact of Manuka honey dressings on the surface pH of chronic wounds. Int Wound J 5:185–194

    Article  PubMed  Google Scholar 

  • Grassberger M, Frank C (2003) Wundheilung durch sterile Fliegenlarven: mechanische, biochemische und mikrobiologische Grundlagen. Schwarz, Urban, München

    Google Scholar 

  • Greener B, Hughes A, Banister NP, Douglass J (2005) Proteases and pH in chronic wounds. J Wound Care 14:59–61

    PubMed  CAS  Google Scholar 

  • Harris LG, Bexfield A, Nigam Y, Rohde H, Ratcliffe NA, Mack D (2009) Disruption of Staphylococcus epidermidis biofilms by medicinal maggot Lucilia sericata excretions/secretions. Int J Artif Organs 32(9):555–564

    PubMed  CAS  Google Scholar 

  • Hobson RP (1931) On an enzyme from blowfly larvae (Lucilia sericata) which digests collagen in alkaline solution. Biochem J 25:1458

    PubMed  CAS  Google Scholar 

  • Hoffman AS (2002) Hydrogels for biomedical applications. Adv Drug Deliv Rev 43:3–12

    Article  Google Scholar 

  • Horobin AJ, Shakesheff KM, Woodrow S, Robinson C, Pritchard DI (2003) Maggots and wound healing: the effect of Lucilia sericata larval secretions upon human dermal fibroblasts. Br J Dermatol 148:923–933

    Article  PubMed  CAS  Google Scholar 

  • Horobin AJ, Shakesheff KM, Pritchard DI (2005) Maggots and wound healing: an investigation of the effects of secretions from Lucilia sericata Larvae upon the migration of human dermal fibroblasts over a fibronectin-coated surface. Wound Rep Reg 13:422–433

    Article  Google Scholar 

  • Huberman L, Gollop N, Mumcuoglu KY, Breuer E, Bhusare SR, Shai Y, Galun R (2007) Antibacterial substances of low molecular weight isolated from the blowfly, Lucilia sericata. Med Vet Entomol 21(2):127–131

    Article  PubMed  CAS  Google Scholar 

  • Jull A, Walker N, Parag V, Molan P, Rodgers A (2008) Randomized clinical trial of honey-impregnated dressings for venous leg ulcers. Br J Surg 95:175–182

    Article  PubMed  Google Scholar 

  • Kerlin RL, East IJ (1991) Potent immunosuppression by secretory/excretory products of larvae from the sheep blowfly Lucilia cuprina. Parasite Immunol 14:595–604

    Article  Google Scholar 

  • Knapp U, Hansis M (1999) Die Wunde. Thieme, Stuttgart, New York

    Google Scholar 

  • König M, Vanscheidt W, Augustin M, Kapp H (2005) Enzymatic versus autolytic debridement of chronic leg ulcers: a prospective randomised trial. J Wound Care 14:320–323

    PubMed  Google Scholar 

  • Lappin-Scott HM (1998) Bacterial–maggot interactions in wound therapy. In: Third international Conference on Biotherapy, Jerusalem, Israel

    Google Scholar 

  • Lee WR, Park JH, Kim KH, Kim SJ, Park DH, Chae MH, Suh SH, Jeong SW, Park KK (2009) The biological effects of tropical alginate treatment in an animal model of skin wound healing. Wound Repair Regen 17(4):505–510

    Article  PubMed  Google Scholar 

  • Light RW, Nguyen T, Mulligan ME, Sasse SA (2000) The in vitro efficacy of varidase versus streptokinase or urokinase for liquefying thick purulent exudative material from loculated empyema. Lung 178(1):13–18

    Article  PubMed  CAS  Google Scholar 

  • Lobmann R, Zemlin C, Motzkau M, Reschke K, Lehnert H (2006) Expression of matrix metalloproteinases and growth factors in diabetic foot wounds treated with a protease absorbent dressing. J Diabetes Complications 20(5):329–335

    Article  PubMed  Google Scholar 

  • Martini E (1946) Medizinische Entomologie. G. Fischer, Jena

    Google Scholar 

  • Mehlhorn H (2008) Encyclopedia of parasitology, 3rd edn. Springer, New York

    Book  Google Scholar 

  • Mehlhorn H, Al-Rasheid KAS, Abdel-Ghaffar F, Klimpel S, Pohle H (2010) Life cycle and attacks of ectoparasites on ruminants during the gear in Central Europe. Parasitol Res 107:425–431

    Article  PubMed  Google Scholar 

  • Mumcuoglu KY (2001) Clinical applications for maggots in wound care. Am J Clin Dermatol 2001(2):219–227

    Article  Google Scholar 

  • Murakami K, Aoki H, Nakamura S, Nakamura S, Takikawa M, Hanzawa M, Kishimoto S, Hattori H, Tanaka Y, Kiyosawa T, Sato Y, Ishihara M (2010) Hydrogel blends of chitin/chitinosan, fucoidan and alginate as healing-impaired wound dressings. Biomaterials 31(1):83–90

    Article  PubMed  CAS  Google Scholar 

  • Nemoto K, Hirota K, Murakami K, Taniguti K, Murata H, Viducic D, Miyake Y (2003) Effect of Varidase (streptodornase) on biofilm formed by Pseudomonas aeruginosa. Chemotherapy 49(3):121–125

    Article  PubMed  CAS  Google Scholar 

  • Patthy L, Trexler M, Váli Z, Baányai L, Váradi A (1984) Kringles: modules specialized for protein binding. Homology of the gelatine-binding region of fibronectin with the kringle structures of proteases. FEBS Lett 171(1):131–136

    Article  PubMed  CAS  Google Scholar 

  • Pecivova J, Macickova T, Takac P, Kovacsova M, Cupanikova D, Kozanek M (2008) Effect of the extract from salivary glands of Lucilia sericata on human neurophils. Neuroendocrinol Lett 29(5):794–797

    PubMed  Google Scholar 

  • Prete PE (1997) Growth effects of Phaenicia sericata larval extracts on fibroblasts: mechanism for wound healing by maggot therapy. Life Sci 60(8):505–510

    Article  PubMed  CAS  Google Scholar 

  • Protz K (2009) Moderne Wundversorgung, 5th edn. Urban & Fischer, München

    Google Scholar 

  • Robinson W, Norwood VH (1933) The role of surgical maggots in the disinfection of osteomyelitis and other infected wounds. J Bone Joint Surg 15:409–412

    Google Scholar 

  • Robson V, Dodd S, Thomas S (2009) Standardized antibacterial honey (Medihoney) with standard therapy in wound care: randomized clinical trial. J Adv Nurs 65(3):565–575

    Article  PubMed  Google Scholar 

  • Romanelli V, Dini V, Bertone MS (2010) Randomized comparison of OASIS wound matrix versus moist wound dressing in the treatment of difficult-to-heal wounds of mixed arterial/venous etiology. Adv Skin Wound Care 23(1):34–38

    Article  PubMed  Google Scholar 

  • Sherman RA (2003) Maggot therapy for treating diabetic foot ulcers unresponsive to conventional therapy. Diabetes Care 2003(26):446–451

    Article  Google Scholar 

  • Sherman RA, Hall MJ, Thomas S (2000) Medicinal maggots: ancient remedy for some contemporary applications. Annu Rev Entomol 45:55–81

    Article  PubMed  CAS  Google Scholar 

  • Smeets R, Ulrich D, Unglaub F, Wöltje M, Pallua N (2008) Effect of oxidised regenerated cellulose/collagen matrix on proteases in wound exudates of patients with chronic venous ulceration. Int Wound J 5:195–203

    Article  PubMed  Google Scholar 

  • Smith AG, Powis RA, Pritchard DI, Britland ST (2006) Greenbottle (Lucilia sericata) larval secretions delivered from a prototype hydrogel wound dressing accelerate the closure of model wounds. Biotechnol Prog 22:1690–1696

    PubMed  CAS  Google Scholar 

  • Takahashi S, Shinya T, Sugiyama A (2010) Angiostatin inhibition of vascular endothelial growth factor-stimulated nitric oxide production in endothelial cells. J Pharmacol Sci 112:432–437

    Article  PubMed  CAS  Google Scholar 

  • Tellam RL, Eisemnn CH, Vuocolo T, Casu R, Jarmey J, Bowles V, Pearson R (2001) Role of oligosaccharides in the immune response of sheep vaccinated with Lucilia cuprina larval glycoprotein, peritrophin-95. Int J Parasitol 8:798–809

    Article  Google Scholar 

  • van der Plas MJ, Jukema GN, Wai SW, Dogterom-Ballering HC, Lagendijk EL, van Gulpen C, van Dissel JT, Bloemberg GV, Nibbering PH (2008) Maggot excretions/secretions are differentially effective against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. J Antimicrob Chemother 61(1):117–122

    Article  PubMed  Google Scholar 

  • van der Plas MJ, van Dissel JT, Nibbering PH (2009) Maggot secretions skew monocyte-macrophage differentiation away from a pro-inflammatory to a proangiogenic type. PLoS One 30;4(11):e8071

    Article  Google Scholar 

  • van der Plas MJ, Dambrot C, Dogterom-Ballering HC, Kruithof S, van Dissel JT, Nibbering PH (2010) Combinations of maggot excretions/secretions and antibiotics are effective against Staphylococcus aureus biofilms and the bacteria derived therefrom. J Antimicrob Chemother 65:917–923

    Article  PubMed  Google Scholar 

  • Wicke C, Halliday B, Allen D, Roche NS, Scheuenstuhl H, Spencer MM, Roberts AB, Hunt TK (2000) Effects of steroids and retinoids on wound healing. Arch Surg 135:1265–1270

    Article  PubMed  CAS  Google Scholar 

  • Wiegand C, Heinze T, Hipler UC (2009a) Comparative in vitro study on cytotoxicity, antimicrobial activity, and binding capacity for pathophysiological factors in chronic wounds of alginate and silver-containing alginate. Wound Repair Regen 17(4):511–521

    Article  PubMed  Google Scholar 

  • Wiegand C, Schönfelder U, Abel M, Ruth O, Kraatz M, Hipler UC (2009b) Protease and pro-inflammatory cytokine concentrations are elevated in chronic compared to acute wounds and can be modulated by collagen type I in vitro. Arch Dermatol Res 302(6):419–428

    Article  PubMed  Google Scholar 

  • Wollina U, Liebold K, Schmidt WD, Hartmann M, Fassler D (2002) Biosurgery supports granulation and debridement in chronic wounds-clinical data and remittance spectroscopy measurement. Int J Dermatol 42:635–639

    Article  Google Scholar 

  • Ziffren SE, Heist HE, May SC, Womack NA (1953) The secretion of collagenase by maggots and its implication. Ann Surg 138:932–934

    Article  PubMed  CAS  Google Scholar 

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Mehlhorn, H., Gestmann, F. (2011). Extracts from Fly Maggots and Fly Pupae as a “Wound Healer”. In: Mehlhorn, H. (eds) Nature Helps.... Parasitology Research Monographs, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19382-8_14

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