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Quantitative Methods for the Estimation of Tannins in Plant Tissues

  • Chapter
Plant Polyphenols

Part of the book series: Basic Life Sciences ((BLSC,volume 59))

Abstract

The different assays used for tannin estimation in plant tissues can be classified into three groups according to the type of reaction involved: precipitation of proteins or alkaloids, reaction with phenolic rings, and depolymerization. The value of these methods for estimation of total tannins or of a particular group of tannins (proanthocyanidins, gallotannins or ellagitannins) is discussed. The importance of careful preparation of the tannin extract, including preparation and storage of the sample, extraction and fractionation of the isolates, is emphasized.

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Authors and Affiliations

  1. Laboratory for Biological Chemistry (INRA), Center of Agro-Industrial Biotechnology, National Agronomy Institute, Paris-Grignon, 78850, Thiverval-Grignon, France

    Augustin Scalbert

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  1. United States Department of Agriculture, Forest Service, Pineville, Louisiana, USA

    Richard W. Hemingway

  2. Institute of Wood Research, Houghton, Michigan, USA

    Peter E. Laks

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Scalbert, A. (1992). Quantitative Methods for the Estimation of Tannins in Plant Tissues. In: Hemingway, R.W., Laks, P.E. (eds) Plant Polyphenols. Basic Life Sciences, vol 59. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3476-1_15

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