Abstract
Inundative mycoherbicides have not been successful in weed control in row crops, probably due to evolutionary barriers, and adding virulence factors was considered essential. Exogenous addition of the products of various geneswas used to ascertain synergy as a prelude to adding them transgenically. Transgenically over-expressing single “soft” genes (host lytic enzymes such as pectinase, cellulase and expansins, or natural hormones such as IAA), or “hard” genes encoding toxins such as NEP1 and CP1, has enhanced virulence, but not enough. Gene stacking to obtain synergies among the various genes is considered a top priority, both to achieve sufficient virulence and to delay the evolution of weed resistance to the fungal pathogens.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
A. Sharon, Z. Amsellem, and J. Gressel, Glyphosate suppression of an elicited defense response, Plant Physiol. 98, 654–659 (1992).
J. Gressel, D. Michaeli, V. Kampel, Z. Amsellem, and A. Warshawsky, Ultralow calcium requirements of fungi facilitate use of calcium regulating agents to suppress host calcium-dependent defenses, synergizing infection by a mycoherbicide, J. Agric. Food Chem. 50, 6353–6360 (2002).
B. Cohen, Z. Amsellem, R. Maor, A. Sharon, and J. Gressel, Transgenically-enhanced expression of indole-3- acetic acid (IAA) confers hypervirulence to plant pathogens, Phytopathology 92, 590–596 (2002).
Z. Amsellem, B. A. Cohen, and J. Gressel, Transgenically conferring sufficient hypervirulence on an inundative mycoherbicidal fungus for efficient weed control, Nat. Biotechnol. 20, 1035–1039 (2002).
L. M. Ciuffetti, R. P. Tuori, and J. M. Gaventa, A single gene encodes a selective toxin causal to the development of tan spot of wheat, Plant Cell 9, 135–144 (1997).
R. G. Atkinson, A cDNA clone for endopolygalacturonase from apple, Plant Physiol. 105, 1437–1438 (1994).
L. Qin, U. Kudla, E. H. A. Roze, A. Goverse, H. Popeijus, J. Nieuwland, H. Overmars, J. T. Jones, A. Schots, G. Smant, J. Bakker, and J. Helder, Plant degradation: A nematode expansin acting on plants, Nature 427, 30 (2004).
S. G. Zhou and L. O. Ingram, Synergistic hydrolysis of carboxymethyl cellulose and acid-swollen cellulose by two endoglucanases (CelZ and CelY) from Erwinia chrysanthemi, J. Bacteriol. 182, 5676–5682 (2000).
B. Bailey, R. Collins, and J. Anderson, Factors influencing the herbicidal activity of Nep1, a fungal protein that induces the hypersensitive response in Centaurea maculosa, Weed Sci. 48, 776–785 (2000).
L. Pazzagli, G. Cappugi, G. Manao, G. Camici, A. Santini, and A. Scala, Purification, characterization, and amino acid sequence of cerato-platanin, a new phytotoxic protein from Ceratocystis fimbriata f. sp. platani, J. Biol. Chem. 274, 24959–24964 (1999).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Gressel, J. et al. (2007). APPROACHES TO AND SUCCESSES IN DEVELOPING TRANSGENICALLY ENHANCED MYCOHERBICIDES. In: Vurro, M., Gressel, J. (eds) Novel Biotechnologies for Biocontrol Agent Enhancement and Management. NATO Security through Science Series. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5799-1_16
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5799-1_16
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5797-7
Online ISBN: 978-1-4020-5799-1
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)