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Biochemical Properties of Recombinant Prolyl Dipeptidases DPP-IV and DPP8

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Dipeptidyl Aminopeptidases

Part of the book series: Advances in Experimental Medicine and Biology ((volume 575))

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References

  • Abbott CA, McCaughan GW and Gorrell MD, 1999, Two highly conserved glutamic acid residues in the predicted beta propeller domain of dipeptidyl peptidase IV are required for its enzyme activity. FEBS Lett. 458: 278–284.

    Article  CAS  PubMed  Google Scholar 

  • Abbott CA, Yu DM, Woollatt E, Sutherland GR, McCaughan GW and Gorrell MD, 2000, Cloning, expression and chromosomal localization of a novel human dipeptidyl peptidase (DPP) IV homolog, DPP8. Eur J Biochem. 267: 6140–6150.

    Article  CAS  PubMed  Google Scholar 

  • Aertgeerts K, Ye S, Shi L, Prasad SG, Witmer D, Chi E, Sang BC, Wijnands RA, Webb DR and Swanson RV, 2004, N-linked glycosylation of dipeptidyl peptidase IV (CD26): effects on enzyme activity, homodimer formation, and adenosine deaminase binding. Protein Sci. 13: 145–154.

    Article  CAS  PubMed  Google Scholar 

  • Aertgeerts K, Ye S, Tennant MG, Kraus ML, Rogers J, Sang BC, Skene RJ, Webb DR and Prasad GS, 2004b, Crystal structure of human dipeptidyl peptidase IV in complex with a decapeptide reveals details on substrate specificity and tetrahedral intermediate formation. Protein Sci. 13: 412–421.

    Article  CAS  PubMed  Google Scholar 

  • Ahren B, Simonsson E, Larsson H, Landin-Olsson M, Torgeirsson H, Jansson PA, Sandqvist M, Bavenholm P, Efendic S, Eriksson JW, Dickinson S and Holmes D, 2002, Inhibition of dipeptidyl peptidase IV improves metabolic control over a 4-week study period in type 2 diabetes. Diabetes Care. 25: 869–875.

    CAS  PubMed  Google Scholar 

  • Bär J, Weber A, Hoffmann T, Stork J, Wermann M, Wagner L, Aust S, Gerhartz B and Demuth HU, 2003, Characterisation of human dipeptidyl peptidase IV expressed in Pichia pastoris. A structural and mechanistic comparison between the recombinant human and the purified porcine enzyme. Biol Chem. 384: 1553–1563.

    Article  PubMed  Google Scholar 

  • Bjelke JR, Christensen J, Branner S, Wagtmann N, Olsen C, Kanstrup AB and Rasmussen HB, 2004, Tyrosine 547 constitutes an essential part of the catalytic mechanism of dipeptidyl peptidase IV. J Biol Chem. 279: 34691–34697.

    Article  CAS  PubMed  Google Scholar 

  • Caporale C, Fontanella A, Petrilli P, Pucci P, Molinaro MF, Picone D and Auricchio, S, 1985, Isolation and characterization of dipeptidyl peptidase IV from human meconium. Functional role of beta-casomorphins. FEBS Lett. 184: 273–277.

    Article  CAS  PubMed  Google Scholar 

  • Chen YS, Chien CH, Goparaju CM, Hsu JT, Liang PH and Chen X, 2004, Purification and characterization of human prolyl dipeptidase DPP8 in Sf9 insect cells. Protein Expr Purif. 35: 142–146.

    Article  CAS  PubMed  Google Scholar 

  • Chien CH, Huang LH, Chou CY, Chen YS, Han YS, Chang GG, Liang PH and Chen X, 2004, One site mutation disrupts dimer formation in human DPP-IV proteins. J Biol Chem. 279: 52338–52345.

    Article  CAS  PubMed  Google Scholar 

  • Conarello SL, Li Z, Ronan J, Roy RS, Zhu L, Jiang G, Liu F, Woods J, Zycband E, Moller DE, Thornberry NA and Zhang BB, 2003, Mice lacking dipeptidyl peptidase IV are protected against obesity and insulin resistance. Proc Natl Acad Sci USA. 100: 6825–6830.

    Article  CAS  PubMed  Google Scholar 

  • Daddona PE and Kelley WN, 1978, Human adenosine deaminase binding protein. Assay, purification, and properties. J Biol Chem. 253: 4617–4623.

    CAS  PubMed  Google Scholar 

  • De Meester I, Vanhoof G, Hendriks D, Demuth HU, Yaron A and Scharpe S, 1992, Characterization of dipeptidyl peptidase IV (CD26) from human lymphocytes. Clin Chim Acta. 210: 23–34.

    Article  PubMed  Google Scholar 

  • De Meester I, Vanhoof G, Lambeir AM and Scharpe S, 1996, Use of immobilized adenosine deaminase (EC 3.5.4.4) for the rapid purification of native human CD26/dipeptidyl peptidase IV (EC 3.4.14.5). J Immunol Methods. 189: 99–105.

    Article  PubMed  Google Scholar 

  • Deacon CF, Wamberg S, Bie P, Hughes TE and Holst JJ, 2002, Preservation of active incretin hormones by inhibition of dipeptidyl peptidase IV suppresses meal-induced incretin secretion in dogs. J Endocrinol. 172: 355–362.

    Article  CAS  PubMed  Google Scholar 

  • Dobers J, Zimmermann-Kordmann M, Leddermann M, Schewe T, Reutter W and Fan H, 2002, Expression, purifi-cation, and characterization of human dipeptidyl peptidase IV/CD26 in Sf9 insect cells. Protein Expr Purif. 25: 527–532.

    Article  CAS  PubMed  Google Scholar 

  • Engel M, Hoffmann T, Wagner L, Wermann M, Heiser U, Kiefersauer R, Huber R, Bode W, Demuth HU and Brandstetter H, 2003, The crystal structure of dipeptidyl peptidase IV (CD26) reveals its functional regulation and enzymatic mechanism. Proc Natl Acad Sci USA. 100: 5063–5068.

    Article  CAS  PubMed  Google Scholar 

  • Hiramatsu H, Kyono K, Higashiyama Y, Fukushima C, Shima H, Sugiyama S, Inaka K, Yamamoto A and Shimizu R, 2003, The structure and function of human dipeptidyl peptidase IV, possessing a unique eight-bladed betapropeller fold. Biochem Biophys Res Commun. 302: 849–854.

    Article  CAS  PubMed  Google Scholar 

  • Hiramatsu H, Yamamoto A, Kyono K, Higashiyama Y, Fukushima C, Shima H, Sugiyama S, Inaka K and Shimizu R, 2004, The crystal structure of human dipeptidyl peptidase IV (DPPIV) complex with diprotin A. Biol Chem. 385: 561–564.

    Article  CAS  PubMed  Google Scholar 

  • Iwaki-Egawa S, Watanabe Y, Kikuya Y and Fujimoto Y, 1998, Dipeptidyl peptidase IV from human serum: purification, characterization, and N-terminal amino acid sequence. J Biochem (Tokyo). 124: 428–433.

    CAS  Google Scholar 

  • Jiaang WT, Chen YS, Hsu T, Wu SH, Chien CH, Chang CN, Chang SP, Lee SJ and Chen X, 2005, Novel isoindoline compounds for potent and selective inhibition of prolyl dipeptidase DPP8. Bioorg Med Chem Lett. 15: 687–691.

    Article  CAS  PubMed  Google Scholar 

  • Kato T, Hama T, Kojima K, Nagatsu T and Sakakibara S, 1978, Excretion of X-prolyl dipeptidyl-aminopeptidase in human urine as determined with a new fluorogenic substrate. Clin Chem. 24: 1163–1166.

    CAS  PubMed  Google Scholar 

  • Lambeir AM, Proost P, Durinx C, Bal G, Senten K, Augustyns K, Scharpe S, Van Damme J and De Meester I, 2001, Kinetic investigation of chemokine truncation by CD26/ dipeptidyl peptidase IV reveals a striking selectivity within the chemokine family. J Biol Chem. 276: 29839–29845.

    Article  CAS  PubMed  Google Scholar 

  • Marguet D, Baggio L, Kobayashi T, Bernard AM, Pierres M, Nielsen PF, Ribel U, Watanabe T, Drucker DJ and Wagtmann N, 2000, Enhanced insulin secretion and improved glucose tolerance in mice lacking CD26. Proc Natl Acad Sci USA. 97: 6874–6879.

    Article  CAS  PubMed  Google Scholar 

  • Mentlein R, 1999, Dipeptidyl-peptidase IV (CD26)-role in the inactivation of regulatory peptides. Regul Pept. 85: 9–24.

    Article  CAS  PubMed  Google Scholar 

  • Mentlein R, Gallwitz B and Schmidt WE, 1993, Dipeptidyl-peptidase IV hydrolyses gastric inhibitory polypeptide, glucagon-like peptide-1(7-36)amide, peptide histidine methionine and is responsible for their degradation in human serum. Eur J Biochem. 214: 829–835.

    Article  CAS  PubMed  Google Scholar 

  • Mitani H, Takimoto M, Hughes TE and Kimura M, 2002, Dipeptidyl peptidase IV inhibition improves impaired glucose tolerance in high-fat diet-fed rats: study using a Fischer 344 rat substrain deficient in its enzyme activity. Jpn J Pharmacol. 88: 442–450.

    Article  CAS  PubMed  Google Scholar 

  • Morimoto C and Schlossman SF, 1998, The structure and function of CD26 in the T-cell immune response. Immunol Rev. 161: 55–70.

    Article  CAS  PubMed  Google Scholar 

  • Oefner C, D’Arcy A, Mac Sweeney A, Pierau S, Gardiner R and Dale GE, 2003, High-resolution structure of human apo dipeptidyl peptidase IV/CD26 and its complex with 1-[([2-[(5-iodopyridin-2-yl)amino]-ethyl]amino)-acetyl]-2-cyano-(S)-pyrrolidine. Acta Crystallogr D Biol Crystallogr. 59: 1206–1212.

    Article  PubMed  Google Scholar 

  • Pospisilik JA, Stafford SG, Demuth HU, McIntosh CH and Pederson RA, 2002, Long-term treatment with dipeptidyl peptidase IV inhibitor improves hepatic and peripheral insulin sensitivity in the VDF Zucker rat: an euglycemichyperinsulinemic clamp study. Diabetes. 51: 2677–2683.

    CAS  PubMed  Google Scholar 

  • Proost P, Menten P, Struyf S, Schutyser E, De Meester I and Van Damme J, 2000, Cleavage by CD26/dipeptidyl peptidase IV converts the chemokine LD78beta into a most efficient monocyte attractant and CCR1 agonist. Blood. 96: 1674–1680.

    CAS  PubMed  Google Scholar 

  • Proost P, Struyf S, Schols D, Durinx C, Wuyts A, Lenaerts JP, De Clercq E, De Meester I and Van Damme J, 1998, Processing by CD26/dipeptidyl-peptidase IV reduces the chemo-tactic and anti-HIV-1 activity of stromal-cellderived factor-1alpha. FEBS Lett. 432: 73–76.

    Article  CAS  PubMed  Google Scholar 

  • Puschel G, Mentlein R and Heymann E, 1982, Isolation and characterization of dipeptidyl peptidase IV from human placenta. Eur J Biochem. 126: 359–365.

    Article  CAS  PubMed  Google Scholar 

  • Rasmussen HB, Branner S, Wiberg FC and Wagtmann N, 2003, Crystal structure of human dipeptidyl peptidase IV/CD26 in complex with a substrate analog. Nat Struct Biol. 10: 19–25.

    Article  CAS  PubMed  Google Scholar 

  • Reimer MK, Holst JJ and Ahren B, 2002, Long-term inhibition of dipeptidyl peptidase IV improves glucose tolerance and preserves islet function in mice. Eur J Endocrinol. 146: 717–727.

    Article  CAS  PubMed  Google Scholar 

  • Richard E, Alam SM, Arredondo-Vega FX, Patel DD and Hershfield MS, 2002, Clustered charged amino acids of human adenosine deaminase comprise a functional epitope for binding the adenosine deaminase complexing protein CD26/dipeptidyl peptidase IV. J Biol Chem. 277: 19720–19726.

    Article  CAS  PubMed  Google Scholar 

  • Rosenblum JS and Kozarich JW, 2003, Prolyl peptidases: a serine protease subfamily with high potential for drug discovery. Curr Opin Chem Biol. 7: 496–504.

    Article  CAS  PubMed  Google Scholar 

  • Saison M, Verlinden J, Van Leuven F, Cassiman JJ and Van den Berghe H, 1983, Identification of cell surface dipeptidylpeptidase IV in human fibroblasts. Biochem J. 216: 177–183.

    CAS  PubMed  Google Scholar 

  • Schrader WP and West CA, 1990, Localization of adenosine deaminase and adenosine deaminase complexing protein in rabbit heart. Implications for adenosine metabolism. Circ Res. 66: 754–762.

    CAS  PubMed  Google Scholar 

  • Thoma R, Loffler B, Stihle M, Huber W, Ruf A and Hennig M, 2003, Structural basis of proline-specific exopeptidase activity as observed in human dipeptidyl peptidase-IV. Structure (Camb). 11: 947–959.

    Article  CAS  Google Scholar 

  • Weihofen WA, Liu J, Reutter W, Saenger W and Fan H, 2004, Crystal structure of CD26/dipeptidyl-peptidase IV in complex with adenosine deaminase reveals a highly amphiphilic interface. J Biol Chem. 279: 43330–43335.

    Article  CAS  PubMed  Google Scholar 

  • Zhu L, Tamvakopoulos C, Xie D, Dragovic J, Shen X, Fenyk-Melody JE, Schmidt K, Bagchi A, Griffin PR, Thornberry NA and Sinha Roy R, 2003, The role of dipeptidyl peptidase IV in the cleavage of glucagon family peptides: in vivo metabolism of pituitary adenylate cyclase activating polypeptide-(1-38). J Biol Chem. 278: 22418–22423.

    Article  CAS  PubMed  Google Scholar 

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Chen, X. (2006). Biochemical Properties of Recombinant Prolyl Dipeptidases DPP-IV and DPP8. In: Lendeckel, U., Reinhold, D., Bank, U. (eds) Dipeptidyl Aminopeptidases. Advances in Experimental Medicine and Biology, vol 575. Springer, Boston, MA . https://doi.org/10.1007/0-387-32824-6_3

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