MAP Kinase Signaling Protocols

1. Front Matter

   Pages i-xi

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2. The ERK Cascade As a Prototype of MAPK Signaling Pathways

   • Hadara Rubinfeld, Rony Seger

   Pages 1-28

3. Determination of ERK Activity

   • Sarah Kraus, Rony Seger

   Pages 29-47

4. Detection of ERK1/2 Activities Using Affinity Reagents

   • Rafael Pulido, Ángel Zúñiga, Axel Ullrich

   Pages 49-60

5. Activation of SAPKs/JNKs and p38s In Vitro

   • John M. Kyriakis, Hong Liu, Deborah N. Chadee

   Pages 61-88

6. Investigating the Cellular BMK1/ERK5 Signaling Pathway

   • Richard I. Tapping, Kato Yutaka, Ta-Hsiang Chao, Masaaki Hayashi, Jeng-Fan Lo, Sung-Woo Kim et al.

   Pages 89-96

7. Pull-Down Assays for Guanosine 5′-Triphosphate-Bound Ras-Like Guanosine 5′-Triphosphatases

   • Miranda van Triest, Johannes L. Bos

   Pages 97-102

8. Regulation of MAPK Cascades by Protein Tyrosine Phosphatases

   • Josema Torres, Carmen Blanco-Aparicio, Rafael Pulido

   Pages 103-112

9. Use of Inhibitors in the Study of MAPK Signaling

   • Yoav D. Shaul, Rony Seger

   Pages 113-125

10. Structure of MAPKs

    • Elizabeth J. Goldsmith, Melanie H. Cobb, Chung-I Chang

    Pages 127-143

11. Subcellular Localization of MAPKs

    • Makoto Adachi, Eisuke Nishida

    Pages 145-153

12. Study of MAPK Signaling Using Knockout Mice

    • Gilles Pagès, Jacques Pouysségur

    Pages 155-166

13. Computer Simulation of MAPK Signal Transduction

    • Baltazar D. Aguda, Herbert M. Sauro

    Pages 167-175

14. Signaling by Growth Factor Receptors

    • Bose S. Kochupurakkal, Yosef Yarden

    Pages 177-202

15. Activation of MAPKs by G Protein-Coupled Receptors

    • Piero Crespo, J. Silvio Gutkind

    Pages 203-210

16. Identification of MAPK Substrates by Expression Screening With Solid-Phase Phosphorylation

    • Rikiro Fukunaga, Tony Hunter

    Pages 211-236

17. Study of Substrate Specificity of MAPKs Using Oriented Peptide Libraries

    • Michael B. Yaffe

    Pages 237-249

18. Ras Signaling Pathway for Analysis of Protein-Protein Interactions in Yeast and Mammalian Cells

    • Ami Aronheim

    Pages 251-261

19. Methods in Functional Proteomics

    • Karine R. Bernard, Karen R. Jonscher, Katheryn A. Resing, Natalie G. Ahn

    Pages 263-281

20. Practical Methods for Deuterium Exchange/Mass Spectrometry

    • Andrew N. Hoofnagle, Katheryn A. Resing, Natalie G. Ahn

    Pages 283-298

Mitogen-activated protein kinase (MAPK) signaling cascades are a group of protein kinases that play a central role in the intracellular transmission of extracellular signals. These cascades operate as major lines of communication within a complicated signaling network that regulates many cellular processes, including proliferation, differentiation, development, stress response, and apoptosis. More than 15,000 papers on MAPKs have been published over the past few years, with the number of publications increasing each year. More and more laboratories embark on the study of MAPK cascades in many d- tinct cellular systems and in particular their role in disease. Future challenges in the study of MAPK cascades remain in understa- ing the role of the various components and isoforms of the cascades in the multiple critical functions that they regulate in the whole organism, as well as the diseases caused by their malfunction. Data from gene-disrupted mice s- gest that inhibition of the MAPK cascades may have serious consequences on the development and growth of the animals. For example, targeted deletion of MEK1 is lethal, owing to developmental problems of placental vasculature and abnormal fibroblast migration. This lethality occurs in spite of the normal expression of MEK2, indicating that although the two MEK isoforms are apparently similar, they do have distinct functions, at least during embryog- esis. The ERK cascade was also shown to play a central role in brain function and in learning and memory.

...this book continues the strong tradition of the series by recruiting the most prominent investigators in the field to provide a detailed discussion of specialized topics with a focus on direct practical applications.-Doody's Health Sciences Book Review Journal

• Department of Biological Regulation, The Zeizmann Institute of Science, Rehovot, Israel

  Rony Seger

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