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Basic Cell Culture Protocols

  • Book
  • © 1997

Overview

Editors:
  1. Jeffrey W. Pollard

    1. Albert Einstein College of Medicine, Bronx

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    You can also search for this editor in PubMed   Google Scholar

  2. John M. Walker

    1. University of Hertfordshire, Hatfield, Herts, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 75)

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Table of contents (37 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Basic Cell Culture

    • Jeffrey W. Pollard
    Pages 1-11

  3. Establishment, Maintenance, and Cloning of Human Dermal Fibroblasts

    • Gareth E. Jones, Clare J. Wise
    Pages 13-21

  4. Aging of Cultured Human Skin Fibroblasts

    • Calvin B. Harley, Steven W. Sherwood
    Pages 23-30

  5. Ex Vivo Maintenance of Differentiated Mammalian Cells

    • Lola M. Reid, Thomas L. Luntz
    Pages 31-57

  6. Scale-Up of Suspension and Anchorage-Dependent Animal Cells

    • Bryan Griffiths, Denis Looby
    Pages 59-75

  7. Hollow-Fiber Cell Culture

    • John M. Davis, Julian A. J. Hanak
    Pages 77-89

  8. Separation and Maintenance of Primary T- and B-Lymphocytes

    • Derek Kinchington, Tony Ng
    Pages 91-100

  9. Human Pilosebaceous Culture

    • Terence Kealey, Michael Philpott, Robert Guy
    Pages 101-115

  10. Keratinocyte Culture

    • Yvonne Barlow, Richard J. Pye
    Pages 117-129

  11. Tissue Culture of Skeletal Muscle

    • Terry A. Partridge
    Pages 131-144

  12. Isolation of Rat Liver Hepatocytes

    • David S. Neufeld
    Pages 145-151

  13. Primary Kidney Cells

    • Mary Taub
    Pages 153-161

  14. Human Thyroid Epithelial Cells

    • D. W. Williams, David Wynford-Thomas
    Pages 163-172

  15. Derivation and Maintenance of Embryonic Stem Cell Cultures

    • Elizabeth J. Robertson
    Pages 173-184

  16. Culture of Cells From Human Tumors of the Nervous System on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cells

    • Manfred Westphal, Alf Giese, Hildegard Meissner, Dorothea Zirkel
    Pages 185-207

  17. Culturing Primitive Hemopoietic Cells

    • Elaine Spooncer, T. Michael Dexter
    Pages 209-219

  18. Collagen Gel Culture of the Human Hematopoietic Progenitors CFU-GM, CFU-E, and BFU-E

    • Annie Allegraud, Irène Dobo, Vincent Praloran
    Pages 221-230

  19. Long-Term B-Lymphoid Cultures from Murine Bone Marrow Establishment and Cloning by Using Stromal Cell Line AC 6.21

    • Cheryl A. Whitlock, Christa E. Muller-Sieburg
    Pages 231-248

  20. Human Long-Term Bone Marrow Culture

    • Brian R. Clark, Catriona Jamieson, Armand Keating
    Pages 249-256
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About this book

It is now seven years since the original Animal Cell Culture volume was published. During this time, techniques have advanced dramatically. The- fore, we have completely revised this edition, including many new chapters and updating old chapters. There has also been a proliferation of methods books covering specific areas of tissue culture. Thus, in this revised edition, we have left out chapters on hybridoma technology because of the many books dealing specifically with this subject. Instead, we have concentrated on the original purpose of this volume now entitled Basic Cell Culture Protocols- which was to provide easy-to-follow protocols for animal cell culture, that hopefully, will be useful to experts and beginners alike. We would like again to acknowledge Dr. Cliff Stanners who taught me (JWP) cell culture, as well as members of the Toronto Somatic Cell Genetics Group for their advice and encouragement, in particular, Drs. L. Siminovitch and V. Ling. Elyse Rizzo's valuable secretarial assistance in compiling this new volume is greatly appreciated. Jeffrey Pollard John M. Walker W. Contents Preface ............................................................................................................. v Contributors ..................................................................................................... xi 1 Basic Cell Culture Jeffrey Pollard .................................................................................. 1 2 Establishment, Maintenance, and Cloning of Human Dermal Fibroblasts Gareth Jones and Clare J. Wise ..................................................

Editors and Affiliations

  • Albert Einstein College of Medicine, Bronx

    Jeffrey W. Pollard

  • University of Hertfordshire, Hatfield, Herts, UK

    John M. Walker

Bibliographic Information

  • Book Title: Basic Cell Culture Protocols

  • Editors: Jeffrey W. Pollard, John M. Walker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896034410

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1997

  • eBook ISBN: 978-1-59259-561-7Published: 01 March 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: 504

  • Topics: Cell Biology

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