Adenovirus Methods and Protocols

Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models

Editors:

ISBN: 978-1-58829-598-9 (Print) 978-1-59745-166-6 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii

  2. No Access

    Book Protocol

    Pages 1-17

    Manipulation of Early Region 4

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    Pages 19-28

    Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants

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    Pages 29-39

    Construction of Adenovirus Type 5 Early Region 1 and 4 Virus Mutants

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    Book Protocol

    Pages 41-59

    Construction of Mouse Adenovirus Type 1 Mutants

  6. No Access

    Book Protocol

    Pages 61-68

    Generation of Recombinant Adenovirus Using the Escherichia coli BJ5183 Recombination System

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    Book Protocol

    Pages 69-90

    Production and Release Testing of Ovine Atadenovirus Vectors

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    Pages 91-106

    Construction of Capsid-Modified Recombinant Bovine Adenovirus Type 3

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    Pages 107-123

    Adenovirus Capsid Chimeras

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    Book Protocol

    Pages 125-134

    Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis

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    Pages 135-144

    Evaluating Apoptosis in Tumor Cells Infected With Adenovirus Expressing p53 Tumor Suppressor Gene

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    Book Protocol

    Pages 145-156

    Growth and Purification of Enteric Adenovirus Type 40

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    Book Protocol

    Pages 157-168

    Immunocompetent, Semi-Permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses

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    Book Protocol

    Pages 169-183

    Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors

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    Book Protocol

    Pages 185-192

    A Real-Time PCR Method to Rapidly Titer Adenovirus Stocks

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    Book Protocol

    Pages 193-204

    Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR

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    Book Protocol

    Pages 205-213

    Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins

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    Book Protocol

    Pages 215-221

    Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs

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    Book Protocol

    Pages 223-235

    Preparation and Titration of CsCl-Banded Adenovirus Stocks

  20. Back Matter

    Pages 237-241