Fluorescent Energy Transfer Nucleic Acid Probes

Designs and Protocols

Editors:

ISBN: 978-1-58829-380-0 (Print) 978-1-59745-069-0 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Design of Energy Transfer Probes

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-16

      Selection of Fluorophore and Quencher Pairs for Fluorescent Nucleic Acid Hybridization Probes

    3. No Access

      Book Protocol

      Pages 17-29

      Choosing Reporter-Quencher Pairs for Efficient Quenching Through Formation of Intramolecular Dimers

  3. Energy Transfer Probes for DNA and RNA Hybridization Detection and Monitoring

    1. Front Matter

      Pages 31-31

    2. No Access

      Book Protocol

      Pages 33-41

      Detection of DNA Hybridization Using Induced Fluorescence Resonance Energy Transfer

    3. No Access

      Book Protocol

      Pages 43-56

      Detecting RNA/DNA Hybridization Using Double-Labeled Donor Probes With Enhanced Fluorescence Resonance Energy Transfer Signals

  4. Energy Transfer Probes for DNA Breaks Detection and DNA Cleavage Monitoring

    1. Front Matter

      Pages 57-57

    2. No Access

      Book Protocol

      Pages 59-69

      Oscillating Probe for Dual Detection of 5'PO4 and 5'OH DNA Breaks in Tissue Sections

    3. No Access

      Book Protocol

      Pages 71-81

      Using Molecular Beacons for Sensitive Fluorescence Assays of the Enzymatic Cleavage of Nucleic Acids

    4. No Access

      Book Protocol

      Pages 83-92

      A Continuous Assay for DNA Cleavage Using Molecular Break Lights

  5. Monitoring of DNA Synthesis and Amplification Using Energy Transfer Probes

    1. Front Matter

      Pages 93-93

    2. No Access

      Book Protocol

      Pages 95-114

      Homogenous Detection of Nucleic Acids Using Self-Quenched Polymerase Chain Reaction Primers Labeled With a Single Fluorophore (LUX™ Primers)

    3. No Access

      Book Protocol

      Pages 115-133

      Use of Self-Quenched, Fluorogenic LUX™ Primers for Gene Expression Profiling

    4. No Access

      Book Protocol

      Pages 135-145

      TaqMan® Reverse Transcriptase-Polymerase Chain Reaction Coupled With Capillary Electrophoresis for Quantification and Identification of bcr-abl Transcript Type

    5. No Access

      Book Protocol

      Pages 147-156

      Quantitative TaqMan® Assay for the Detection and Monitoring of Cytomegalovirus Infection in Organ Transplant Patients

    6. No Access

      Book Protocol

      Pages 157-169

      Real-Time Detection and Quantification of Telomerase Activity Utilizing Energy Transfer Primers

  6. DNA Sequence Analysis and Mutation Detection Using Fluorescence Energy Transfer

    1. Front Matter

      Pages 171-171

    2. No Access

      Book Protocol

      Pages 173-186

      Invader® Assay for Single-Nucleotide Polymorphism Genotyping and Gene Copy Number Evaluation

    3. No Access

      Book Protocol

      Pages 187-200

      Real-Time Quantitative Polymerase Chain Reaction Analysis of Mitochondrial DNA Point Mutation

    4. No Access

      Book Protocol

      Pages 201-214

      Multiplex Single-Nucleotide Polymorphism Detection by Combinatorial Fluorescence Energy Transfer Tags and Molecular Affinity

    5. No Access

      Book Protocol

      Pages 215-240

      High-Throughput Genotyping With Energy Transfer-Labeled Primers

  7. Determination of Distance and DNA Folding

    1. Front Matter

      Pages 241-241

    2. No Access

      Book Protocol

      Pages 243-255

      Distance Determination in Protein-DNA Complexes Using Fluorescence Resonance Energy Transfer

    3. No Access

      Book Protocol

      Pages 257-271

      Multi-Fluorophore Fluorescence Resonance Energy Transfer for Probing Nucleic Acids Structure and Folding

  8. Dna-Based Biosensors Utilizing Energy Transfer

    1. Front Matter

      Pages 273-273

    2. No Access

      Book Protocol

      Pages 275-288

      Fluorescent DNAzyme Biosensors for Metal Ions Based on Catalytic Molecular Beacons

    3. No Access

      Book Protocol

      Pages 289-310

      Fluorescent Energy Transfer Readout of an Aptazyme-Based Biosensor

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