2005

Molecular Diagnostics

For the Clinical Laboratorian

Editors:

ISBN: 978-1-58829-356-5 (Print) 978-1-59259-928-8 (Online)

Table of contents (44 chapters)

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  1. Front Matter

    Pages i-xvi

  2. Introduction

    1. Front Matter

      Pages 1-1

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      Book Chapter

      Pages 3-10

      An Historical Perspective on the Clinical Diagnostic Laboratory

  3. Basic Molecular Biology

    1. Front Matter

      Pages 11-11

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      Book Chapter

      Pages 13-24

      An Overview of Nucleic Acid Chemistry, Structure, and Function

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      Book Chapter

      Pages 25-30

      Extraction of Nucleic Acids

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      Book Chapter

      Pages 31-46

      Nucleic Acid Blotting Techniques

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      Book Chapter

      Pages 47-55

      The Polymerase Chain Reaction

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      Book Chapter

      Pages 57-61

      Bioinformatics

  4. Molecular Diagnostic Technologies

    1. Front Matter

      Pages 63-63

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      Book Chapter

      Pages 65-74

      PCR-Based Methods for Mutation Detection

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      Book Chapter

      Pages 75-84

      Alternative Methods for Amplified Nucleic Acid Testing

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      Book Chapter

      Pages 85-109

      Electrophoretic Methods for Mutation Detection and DNA Sequencing

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      Book Chapter

      Pages 111-120

      Single-Nucleotide Polymorphisms

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      Book Chapter

      Pages 121-148

      Microarray Approaches to Gene Expression Analysis

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      Book Chapter

      Pages 149-160

      Methods for Analysis of DNA Methylation

  5. Other Clinical Diagnostic Technologies

    1. Front Matter

      Pages 161-161

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      Book Chapter

      Pages 163-172

      Flow Cytometry

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      Book Chapter

      Pages 173-187

      Medical Cytogenetics

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      Book Chapter

      Pages 189-202

      Fluorescence In Situ Hybridization

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      Book Chapter

      Pages 203-217

      Immunohistochemistry

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      Book Chapter

      Pages 219-224

      Laser Capture Microdissection

  6. Quality Assurance in the Molecular Diagnostics Laboratory

    1. Front Matter

      Pages 225-225

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      Book Chapter

      Pages 227-236

      Framework for Quality Assurance in Molecular Diagnostics

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      Book Chapter

      Pages 237-241

      Verification of Molecular Assays

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      Book Chapter

      Pages 243-246

      Standards and Standardization of Molecular Diagnostics

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