Therapeutic Proteins

Methods and Protocols

Editors:

ISBN: 978-1-58829-390-9 (Print) 978-1-59259-922-6 (Online)

Table of contents (35 protocols)

previous Page of 2
  1. Front Matter

    Pages i-xiv

  2. No Access

    Book Protocol

    Pages 1-16

    Biopharmaceutical Proteins

  3. No Access

    Book Protocol

    Pages 17-30

    Expression of Antibody Fragments by Periplasmic Secretion in Escherichia coli

  4. No Access

    Book Protocol

    Pages 31-41

    Secretory Production of Therapeutic Proteins in Escherichia coli

  5. No Access

    Book Protocol

    Pages 43-49

    Expression of Recombinant LTB Protein in Marine vibrio VSP60

  6. No Access

    Book Protocol

    Pages 51-64

    Heterologous Gene Expression in Yeast

  7. No Access

    Book Protocol

    Pages 65-76

    Pharmaceutical Proteins From Methylotrophic Yeasts

  8. No Access

    Book Protocol

    Pages 77-86

    Expression of Human Papillomavirus Type 16 L1 in Baculovirus Expression Systems

  9. No Access

    Book Protocol

    Pages 87-98

    Large-Scale Transient Expression of Therapeutic Proteins in Mammalian Cells

  10. No Access

    Book Protocol

    Pages 99-106

    Site-Specific Integration for High-Level Protein Production in Mammalian Cells

  11. No Access

    Book Protocol

    Pages 107-121

    Production of Recombinant Therapeutic Proteins by Mammalian Cells in Suspension Culture

  12. No Access

    Book Protocol

    Pages 123-143

    Transgene Control Engineering in Mammalian Cells

  13. No Access

    Book Protocol

    Pages 145-154

    Fusion to Albumin as a Means to Slow the Clearance of Small Therapeutic Proteins Using the Pichia pastoris Expression System

  14. No Access

    Book Protocol

    Pages 155-161

    High-Throughput Recovery of Therapeutic Proteins from the Inclusion Bodies of Escherichia coli

  15. No Access

    Book Protocol

    Pages 163-176

    Isolation, Solubilization, Refolding, and Chromatographic Purification of Human Growth Hormone from Inclusion Bodies of Escherichia coli Cells

  16. No Access

    Book Protocol

    Pages 177-190

    Large-Scale Preparation of Factor VIIa from Human Plasma

  17. No Access

    Book Protocol

    Pages 191-208

    Purification of Clinical-Grade Monoclonal Antibodies by Chromatographic Methods

  18. No Access

    Book Protocol

    Pages 209-219

    Virus Elimination and Validation

  19. No Access

    Book Protocol

    Pages 221-231

    Virus Removal by Nanofiltration

  20. No Access

    Book Protocol

    Pages 233-242

    Determining Residual Host Cell Antigen Levels in Purified Recombinant Proteins by Slot Blot and Scanning Laser Densitometry

  21. No Access

    Book Protocol

    Pages 243-263

    Principles of Biopharmaceutical Protein Formulation

previous Page of 2