Multiple Myeloma

Methods and Protocols

Editors:

ISBN: 978-1-58829-392-3 (Print) 978-1-59259-916-5 (Online)

Table of contents (23 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-4

    Multiple Myeloma

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    Pages 5-24

    Immunophenotyping of Plasma Cells in Multiple Myeloma

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    Pages 25-35

    Plasma Cell Labeling Index

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    Pages 37-48

    Conventional Cytogenetics in Myeloma

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    Pages 49-58

    Multicolor Spectral Karyotyping in Multiple Myeloma

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    Pages 59-69

    Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization

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    Pages 71-83

    Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma

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    Pages 85-103

    Southern Blotting of IgH Rearrangements in B-Cell Disorders

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    Pages 105-119

    Identification and Assembly of V Genes as Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma

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    Pages 121-144

    Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma

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    Pages 145-163

    Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloma

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    Pages 165-173

    Incomplete DJH Rearrangements

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    Pages 175-181

    Identification of Malignant Plasma Cells by mRNA In Situ Hybridization

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    Pages 183-190

    The SCID-hu Myeloma Model

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    Pages 191-205

    The 5T2MM Murine Model of Multiple Myeloma

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    Pages 207-223

    Determination of Telomerase Activity and Telomere Length

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    Pages 225-234

    Dendritic Cell/Myeloma Hybrid Vaccine

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    Pages 235-244

    Genetically Engineered Myeloma Cell Vaccine

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    Pages 245-256

    An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors

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    Pages 257-267

    Clonality Detection of Expanded T-Cell Populations in Patients With Multiple Myeloma

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