Human Retrovirus Protocols

Virology and Molecular Biology

Editors:

ISBN: 978-1-58829-495-1 (Print) 978-1-59259-907-3 (Online)

Table of contents (37 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Methods for Virus Isolation and Detection

    1. Front Matter

      Pages 2-2

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      Pages 3-15

      Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals

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      Pages 17-24

      Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals

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      Pages 25-33

      Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes

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      Pages 35-48

      Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived Macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid

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      Pages 49-70

      Isolation and HIV-1 Infection of Primary Human Microglia From Fetal and Adult Tissue

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      Pages 71-86

      Isolation of Human Immunodeficiency Virus Type 1 From Semen and Vaginal Fluids

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      Pages 87-94

      Isolation and Quantification of HIV From Lymph Nodes

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      Pages 95-112

      Isolation of Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes

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      Pages 113-123

      Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells

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      Pages 125-137

      Isolation of Foamy Viruses From Peripheral Blood Lymphocytes

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      Pages 139-154

      Alu-LTR Real-Time Nested PCR Assay for Quantifying Integrated HIV-1 DNA

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      Pages 155-170

      Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR

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      Pages 171-182

      Detection of HIV-1 Provirus and RNA by In Situ Amplification

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      Pages 183-189

      Detection of HTLV-1 Gene on Cytologic Smear Slides

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      Pages 191-200

      Detection of HIV-2 by PCR

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      Pages 201-213

      Quantitation of HIV-1 Viral RNA in Blood Plasma and Genital Secretions

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      Pages 215-220

      Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR

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      Pages 221-228

      Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR

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      Pages 229-243

      A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay

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      Pages 245-255

      A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity

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