2005

The Proteomics Protocols Handbook

Editors:

ISBN: 978-1-58829-343-5 (Print) 978-1-59259-890-8 (Online)

Table of contents (73 protocols)

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  1. Front Matter

    Pages i-xviii

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    Pages 1-18

    Extraction and Solubilization of Proteins for Proteomic Studies

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    Pages 19-26

    Preparation of Bacterial Samples for 2-D PAGE

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    Pages 27-30

    Preparation of Yeast Samples for 2-D PAGE

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    Pages 31-35

    Preparation of Mammalian Tissue Samples for Two-Dimensional Electrophoresis

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    Pages 37-48

    Differential Detergent Fractionation of Eukaryotic Cells

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    Pages 49-54

    Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis

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    Pages 55-57

    Preparation of Plant Protein Samples for 2-D PAGE

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    Pages 59-66

    Laser-Assisted Microdissection in Proteomic Analyses

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    Pages 67-78

    Purification of Cellular and Organelle Populations by Fluorescence-Activated Cell Sorting for Proteome Analysis

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    Pages 79-85

    Purification of Nucleoli From Lymphoma Cells and Solubilization of Nucleolar Proteins for 2-DE Separation

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    Pages 87-95

    Prefractionation of Complex Protein Mixture for 2-D PAGE Using Reversed-Phase Liquid Chromatography

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    Pages 97-117

    Fractionation of Complex Proteomes by Microscale Solution Isoelectrofocusing Using ZOOM− IEF Fractionators to Improve Protein Profiling

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    Pages 119-131

    Large-Format 2-D Polyacrylamide Gel Electrophoresis

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    Pages 133-144

    Analysis of Membrane Proteins by Two-Dimensional Gels

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    Pages 145-149

    2-D PAGE of High-Molecular-Mass Proteins

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    Pages 151-165

    Using Ultra-Zoom Gels for High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis

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    Pages 167-175

    NEpHGE and pI Strip Proteomic 2-D Gel Electrophoretic Mapping of Lipid-Rich Membranes

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    Pages 177-184

    Silver Staining of 2-D Gels

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    Pages 185-192

    Zn2+Reverse Staining Technique

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    Pages 193-200

    Multiplexed Proteomics Technology for the Fluorescence Detection of Glycoprotein Levels and Protein Expression Levels Using Pro-Q® Emerald and SYPRO® Ruby Dyes

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