2005

Medical Biomethods Handbook

Editors:

ISBN: 978-1-58829-288-9 (Print) 978-1-59259-870-0 (Online)

Table of contents (44 protocols)

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  1. Front Matter

    Pages i-xv

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    Pages 1-12

    Basic Techniques in Molecular Biology

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    Pages 13-23

    Probe Design, Production, and Applications

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    Pages 25-34

    Restriction Enzymes

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    Pages 35-42

    Southern Blotting as a Diagnostic Method

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    Pages 43-62

    Western Blotting as a Diagnostic Method

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    Pages 63-72

    Principles and Medical Applications of the Polymerase Chain Reaction

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    Pages 73-77

    Single-Strand Conformation Polymorphism (SSCP) Analysis

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    Pages 79-86

    Denaturing Gradient Gel Electrophoresis (DGGE)

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    Pages 87-94

    Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing

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    Pages 95-116

    Introduction to Capillary Electrophoresis of DNA

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    Pages 117-133

    Mapping Techniques

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    Pages 135-145

    Dideoxyfingerprinting for Mutation Detection

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    Pages 147-153

    Conformation-Sensitive Gel Electrophoresis

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    Pages 155-165

    Amplification Refractory Mutation System and Molecular Diagnostics

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    Pages 167-181

    Ligase Chain Reaction

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    Pages 183-194

    Chemical Cleavage of Mismatch

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    Pages 195-204

    The Protein Truncation Test

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    Pages 205-226

    Linkage, Allele Sharing, and Association

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    Pages 227-254

    Single-Nucleotide Polymorphisms

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    Pages 255-271

    cDNA Microarrays

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