Adoptive Immunotherapy: Methods and Protocols

Editors:

ISBN: 978-1-58829-406-7 (Print) 978-1-59259-862-5 (Online)

Table of contents (32 protocols)

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  1. Front Matter

    Pages i-xvi

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    Pages 1-18

    Exploiting Dendritic Cells for Active Immunotherapy of Cancer and Chronic Infection

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    Pages 19-33

    A Mathematical Approach for Optimizing Dendritic Cell-Based Immunotherapy

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    Pages 35-46

    Delivery of Tumor Antigens to Dendritic Cells Using Biodegradable Microspheres

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    Pages 47-54

    RNA Transfection of Dendritic Cells

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    Pages 55-70

    Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System

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    Pages 71-81

    Generation of Clinical-Grade Monocyte-Derived Dendritic Cells Using the CliniMACS System

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    Pages 83-95

    Adenoviral Transduction of Dendritic Cells

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    Pages 97-112

    Generation of Autologous Peptide- and Protein-Pulsed Dendritic Cells for Patient-Specific Immunotherapy

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    Pages 113-125

    Phenotypical and Functional Characterization of Clinical-Grade Dendritic Cells

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    Pages 127-135

    Dendritic Cells in Clinical Trials for Multiple Myeloma

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    Pages 137-154

    Identification of Tumor-Associated Autoantigens With SEREX

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    Pages 155-173

    Application of Proteomics and Protein Analysis for Biomarker and Target Finding for Immunotherapy

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    Pages 175-184

    Isolation and Expansion of Tumor-Reactive Cytotoxic T-Cell Clones for Adoptive Immunotherapy

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    Pages 185-200

    Tracking Adoptively Transferred Antigen-Specific T-Cells With Peptide/MHC Multimers

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    Pages 201-213

    Gene Transfer of MHC-Restricted Receptors

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    Pages 215-228

    Immunotherapy With CTL Restricted by Nonself MHC

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    Pages 229-256

    Designing TCR for Cancer Immunotherapy

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    Pages 257-264

    Isolation and Expansion of Tumor-Specific CD4+T-Cells by Means of Cytokine Secretion

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    Pages 265-283

    Methods for the Ex Vivo Characterization of Human CD8+T Subsets Based on Gene Expression and Replicative History Analysis

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    Pages 285-296

    Regulatory T-Cells in Antitumor Therapy Isolation and Functional Testing of CD4+CD25+Regulatory T-Cells

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