DNA Viruses

Methods and Protocols

Editors:

ISBN: 978-1-58829-353-4 (Print) 978-1-59259-848-9 (Online)

Table of contents (30 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Viral Detection

    1. Front Matter

      Pages 1-81

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      Pages 3-13

      Viral Detection

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      Pages 15-25

      Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1

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      Pages 27-37

      Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR

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      Pages 39-56

      Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In Vivo

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      Pages 57-71

      Detection and Quantification of the Rare Latently Infected Cell Undergoing Herpes Simplex Virus Transcriptional Activation in the Nervous System In Vivo

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      Pages 73-81

      Reporter Cell Lines for the Detection of Herpes Simplex Viruses

  3. Virus Structure And Imaging

    1. Front Matter

      Pages 83-171

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      Pages 85-108

      Unraveling the Architecture of Viruses by High-Resolution Atomic Force Microscopy

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      Pages 109-128

      Studying the Structure of Large Viruses With Multiresolution Imaging

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      Pages 129-142

      Herpes Simplex Virus-Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence

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      Pages 143-160

      Herpes Simplex Virus-Cell Interactions Studied by Immunogold Cryosection Electron Microscopy

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      Pages 161-172

      FTIR Microscopy Detection of Cells Infected With Viruses

  4. Virus Entry

    1. Front Matter

      Pages 173-211

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      Pages 175-185

      The JC Virus-Like Particle Overlay Assay

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      Pages 187-195

      Analysis of Fusion Using a Virus-Free Cell Fusion Assay

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      Pages 197-211

      Pseudovirions as Specific Tools for Investigation of Virus Interactions With Cells

  5. Gene Expression

    1. Front Matter

      Pages 213-244

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      Pages 215-230

      Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence

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      Pages 231-244

      Identification and Characterization of Herpesviral Immediate-Early Genes

  6. Replication And Genome Maintenance

    1. Front Matter

      Pages 245-276

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      Pages 247-265

      Methods for Measuring the Replication and Segregation of Epstein-Barr Virus-Based Plasmids

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      Pages 267-276

      DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins

  7. Pathogenesis

    1. Front Matter

      Pages 277-295

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      Pages 279-283

      Pre-B-Cell Colony Formation Assay

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      Pages 285-294

      Luciferase Real-Time Bioluminescence Imaging for the Study of Viral Pathogenesis

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