Developmental Biology Protocols

Volume I

Editors:

ISBN: 978-0-89603-852-3 (Print) 978-1-59259-685-0 (Online)

Table of contents (51 protocols)

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  1. Front Matter

    Pages i-xxv

  2. Introduction

    1. Front Matter

      Pages 1-1

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      Pages 3-5

      Developmental Biology Protocols

  3. Systems: Production, Culture, and Storage

    1. Front Matter

      Pages 7-7

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      Pages 9-15

      Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies

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      Pages 17-23

      Large-Scale Culture and Preparation of Sea Urchin Embryos for Isolation of Transcriptional Regulatory Proteins

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      Pages 25-29

      The Chick Embryo as a Model System for Analyzing Mechanisms of Development

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      Pages 31-38

      Culture of Avian Embryos

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      Pages 39-46

      Exo Ovo Culture of Avian Embryos

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      Pages 47-52

      Culture of Preimplantation Mouse Embryos

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      Pages 53-76

      In Vitro Culture of Rodent Embryos During the Early Postimplantation Period

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      Pages 77-82

      Cryopreservation of Mouse Embryos

  4. Developmental Pattern and Morphogenesis

    1. Front Matter

      Pages 83-83

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      Pages 85-88

      Studying Head and Brain Development in Drosophila

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      Pages 89-112

      Bioassays of Inductive Interactions in Amphibian Development

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      Pages 113-125

      Gastrulation and Early Mesodermal Patterning in Vertebrates

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      Pages 127-137

      Craniofacial Development of Avian and Rodent Embryos

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      Pages 139-150

      Examination of the Axial Skeleton of Fetal Rodents

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      Pages 151-161

      Cardiac Morphogenesis and Dysmorphogenesis

  5. Embryo Structure and Function

    1. Front Matter

      Pages 163-163

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      Pages 165-173

      Application of Plastic Embedding for Sectioning Whole-Mount Immunostained Early Vertebrate Embryos

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      Pages 175-182

      Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos

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      Pages 183-189

      Whole-Mount Immunolabeling of Embryos by Microinjection

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      Pages 191-202

      Confocal Laser Scanning Microscopy Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos

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      Pages 203-209

      Embryo/Fetal Topographical Analysis by Fluorescence Microscopy and Confocal Laser Scanning Microscopy

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