Adrenergic Receptor Protocols

Editors:

ISBN: 978-0-89603-602-4 (Print) 978-1-59259-684-3 (Online)

Table of contents (35 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Genetic Analysis

    1. Front Matter

      Pages 1-1

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      Pages 3-35

      Construction of Libraries for Isolation of Adrenergic Receptor Genes

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      Pages 37-51

      Isolation of Adrenergic Receptor Genes

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      Pages 53-72

      Analyses of Adrenergic Receptor Sequences

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      Pages 73-89

      Polymerase Chain Reaction Screening of Genomic Libraries for Adrenergic Receptor Genes

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      Pages 91-116

      Solution-Phase Library Screening for Identification of Rare Adrenergic Receptor Clones

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      Pages 117-126

      Genetic Polymorphisms of Adrenergic Receptors

  3. RNA Analysis

    1. Front Matter

      Pages 127-127

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      Pages 129-137

      Northern Blot Analyses Detecting Adrenergic Receptor mRNAs

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      Pages 139-153

      Ribonuclease Protection Assay for the Detection of β1-Adrenergic Receptor RNA

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      Pages 155-168

      Determination of Adrenergic Receptor mRNAs by Quantitative Reverse Transcriptase-Polymerase Chain Reactions

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      Pages 169-180

      Nuclear Run-On Assays for Measurement of Adrenergic Receptor Transcription Rate

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      Pages 181-185

      Primer Extension Methods for Determination of β1-Adrenergic Receptor mRNA Start Sites

  4. Expression Analysis

    1. Front Matter

      Pages 187-187

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      Pages 189-206

      Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors

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      Pages 207-214

      Expression of β-Adrenergic Receptors in Recombinant Baculovirus-Infected Insect Cells

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      Pages 215-220

      Expression of β-Adrenergic Receptors in E. coli

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      Pages 221-234

      Use and Pharmacological Analysis of Established and Transfected Cell Lines Expressing Adrenergic Receptors

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      Pages 235-239

      Transient Transfection and Adrenergic Receptor Promoter Analysis

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      Pages 241-258

      Antisense RNA/DNA-Based Techniques to Probe Adrenergic Receptor Function

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      Pages 259-277

      Targeted Disruption of Adrenergic Receptor Genes

  5. Receptor and Second Messenger Analysis

    1. Front Matter

      Pages 279-279

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      Pages 281-299

      Development of Antibodies to Adrenergic Receptors

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      Pages 301-314

      Western Blot Detection of Adrenergic Receptors

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