Molecular Methods in Developmental Biology

Xenopus and Zebrafish

Editors:

ISBN: 978-0-89603-790-8 (Print) 978-1-59259-678-2 (Online)

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii

  2. No Access

    Book Protocol

    Pages 1-13

    The Animal Cap Assay

  3. No Access

    Book Protocol

    Pages 15-28

    Cell and Tissue Transplantation in Zebrafish Embryos

  4. No Access

    Book Protocol

    Pages 29-40

    Ribonuclease Protection Analysis of Gene Expression in Xenopus

  5. No Access

    Book Protocol

    Pages 41-56

    Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)

  6. No Access

    Book Protocol

    Pages 57-67

    Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos

  7. No Access

    Book Protocol

    Pages 69-76

    In Situ Hybridization to Sections of Xenopus Embryos

  8. No Access

    Book Protocol

    Pages 77-88

    Zebrafish Immunohistochemistry

  9. No Access

    Book Protocol

    Pages 89-97

    Immunohistochemistry of Xenopus Embryos

  10. No Access

    Book Protocol

    Pages 99-109

    Preparation and Testing of Synthetic mRNA for Microinjection

  11. No Access

    Book Protocol

    Pages 111-123

    Microinjection into Xenopus Oocytes and Embryos

  12. No Access

    Book Protocol

    Pages 125-132

    Microinjection into Zebrafish Embryos

  13. No Access

    Book Protocol

    Pages 133-153

    Expression from DNA Injected into Xenopus Embryos

  14. No Access

    Book Protocol

    Pages 155-166

    Promoter Analysis in Zebrafish Embryos

  15. No Access

    Book Protocol

    Pages 167-173

    Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs

  16. No Access

    Book Protocol

    Pages 175-186

    Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis

  17. No Access

    Book Protocol

    Pages 187-198

    DNA-Footprinting using Crude Embryonic Extracts from Xenopus laevis

  18. No Access

    Book Protocol

    Pages 199-212

    Mapping Protein-DNA Interactions Using In Vivo Footprinting

  19. Back Matter

    Pages 213-217