Antibody Engineering

Methods and Protocols

Editors:

ISBN: 978-1-58829-092-2 (Print) 978-1-59259-666-9 (Online)

Table of contents (32 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Antibody Sequence And Structure

    1. Front Matter

      Pages 1-1

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      Pages 3-9

      Internet Resources for the Antibody Engineer

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      Pages 11-25

      The Kabat Database and a Bioinformatics Example

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      Pages 27-49

      IMGT, The International ImMunoGeneTics Information System®, http://imgt.cines.fr

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      Pages 51-91

      Antibody Variable Regions

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      Pages 93-113

      Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling

  3. Antibody-Lead Generation

    1. Front Matter

      Pages 115-115

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      Pages 117-134

      PCR Cloning of Human Immunoglobulin Genes

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      Pages 135-159

      Antibody Humanization by CDR Grafting

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      Pages 161-176

      Selection of Human Antibodies from Phage Display Libraries

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      Pages 177-189

      Production of Human Single-Chain Antibodies by Ribosome Display

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      Pages 191-200

      Production of Human Antibodies from Transgenic Mice

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      Pages 201-208

      Selection of Internalizing Antibodies for Drug Delivery

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      Pages 209-225

      Engineering Multivalent Antibody Fragments for In Vivo Targeting

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      Pages 227-242

      Production of Recombinant Bispecific Antibodies

  4. Antibody Expression And Optimization

    1. Front Matter

      Pages 243-243

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      Pages 245-254

      Expression and Isolation of Recombinant Antibody Fragments in E. coli

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      Pages 255-268

      Expression of Recombinant Antibodies in Mammalian Cell Lines

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      Pages 269-299

      Human Antibody Production Using Insect-Cell Expression Systems

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      Pages 301-318

      Antibody Production in Transgenic Plants

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      Pages 319-326

      Directed Mutagenesis of Antibody Variable Domains

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      Pages 327-343

      Antibody Affinity Maturation by Chain Shuffling

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      Pages 345-359

      Antibody Affinity Maturation by Random Mutagenesis

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