Book Volume 226 2003

PCR Protocols

Editors:

ISBN: 978-0-89603-642-0 (Print) 978-1-59259-384-2 (Online)

Table of contents (71 protocols)

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  1. Front Matter

    Pages i-xvii

  2. Introduction to PCR

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-6

      A Short History of the Polymerase Chain Reaction

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      Protocol

      Pages 7-14

      PCR Patent Issues

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      Protocol

      Pages 15-19

      Equipping and Establishing a PCR Laboratory

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      Protocol

      Pages 21-23

      Quality Control in PCR

  3. Preparation of Nucleic Acid Templates

    1. Front Matter

      Pages 25-25

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      Protocol

      Pages 27-28

      Extraction of Nucleic Acid Templates

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      Protocol

      Pages 29-31

      Extraction of DNA from Whole Blood

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      Protocol

      Pages 33-34

      DNA Extraction from Tissue

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      Protocol

      Pages 35-41

      Extraction of DNA from Microdissected Archival Tissues

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      Protocol

      Pages 43-44

      RNA Extraction from Blood

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      Protocol

      Pages 45-46

      RNA Extraction from Frozen Tissue

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      Protocol

      Pages 47-48

      RNA Extraction from Tissue Sections

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      Protocol

      Pages 49-51

      Dual DNA/RNA Extraction

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      Protocol

      Pages 53-54

      DNA Extraction from Fungi, Yeast, and Bacteria

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      Protocol

      Pages 55-56

      Isolation of RNA Viruses from Biological Materials

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      Protocol

      Pages 57-61

      Extraction of Ancient DNA

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      Protocol

      Pages 63-63

      DNA Extraction from Plasma and Serum

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      Protocol

      Pages 65-75

      Technical Notes for the Detection of Nucleic Acids

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      Protocol

      Pages 77-78

      Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels

  4. Basic PCR Methods

    1. Front Matter

      Pages 79-79

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      Protocol

      Pages 81-88

      PCR Primer Design

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      Protocol

      Pages 89-99

      Optimization of Polymerase Chain Reactions

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