PCR Detection of Microbial Pathogens

Editors:

ISBN: 978-1-58829-049-6 (Print) 978-1-59259-344-6 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xi

  2. Reviews

    1. Front Matter

      Pages 1-1

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      Book Protocol

      Pages 3-29

      Specificity and Performance of Diagnostic PCR Assays

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      Pages 31-50

      Pre-PCR Processing of Samples

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      Pages 51-64

      Critical Aspects of Standardization of PCR

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      Pages 65-84

      PCR Technology and Applications to Zoonotic Food-Borne Bacterial Pathogens

  3. Protocols

    1. Front Matter

      Pages 85-1

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      Book Protocol

      Pages 87-95

      Detection, Identification, and Subtyping of Actinobacillus pleuropneumoniae

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      Pages 97-108

      Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR

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      Pages 109-121

      Isolation of Campylobacter and Identification byPCR

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      Pages 123-136

      Detection and Differentiation of Chlamydiae by Nested PCR

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      Pages 137-152

      Detection of Toxigenic Clostridia

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      Pages 153-161

      PCR-Based Detection of Coxiella burnetii from Clinical Samples

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      Pages 163-184

      Detection and Subtyping of ShigaToxin-Producing Escherichia coli (STEC)

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      Pages 185-192

      Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay

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      Pages 193-200

      Detection of Leptospira interrogans

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      Pages 201-221

      Detection of Pathogenic Mycobacteria of Veterinary Importance

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      Pages 223-230

      Multiplex PCR of Avian Pathogenic Mycoplasmas

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      Pages 231-245

      Detection and Differentiation of Ruminant Mycoplasmas

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      Pages 247-256

      Detection of Mycoplasma hyopneumoniae from Clinical Samples and Air

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      Pages 257-274

      PCR-Detection of Hemophilus paragallinarum, Hemophilus somnus, Mannheimia (Pasteurella) hemolytica, Mannheimia spp., Pasteurella trehalosi, and Pasteurella multocida

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      Book Protocol

      Pages 275-287

      Detection of Salmonella spp.

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      Pages 289-298

      Detection of Toxoplasma gondii

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