E. coli

Shiga Toxin Methods and Protocols

Editors:

ISBN: 978-0-89603-939-1 (Print) 978-1-59259-316-3 (Online)

Table of contents (25 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-7

    The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections

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    Pages 9-26

    Methods for Detection of STEC in Humans

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    Pages 27-43

    Serological Methods for the Detection of STEC Infections

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    Pages 45-54

    Detection and Characterization of STEC in Stool Samples Using PCR

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    Pages 55-65

    Molecular Typing Methods for STEC

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    Pages 67-74

    STEC in the Food Chain

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    Pages 75-89

    STEC as a Veterinary Problem

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    Pages 91-98

    Cellular Microbiology of STEC Infections

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    Pages 99-112

    Analysis of Pathogenicity Islands of STEC

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    Pages 113-124

    Generation of Isogenic Deletion Mutants of STEC

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    Pages 125-135

    Generation of Monoclonal Antibodies Against Secreted Proteins of STEC

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    Pages 137-149

    Microscopic Methods to Study STEC

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    Pages 151-163

    Detection and Characterization of EHEC-Hemolysin

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    Pages 165-186

    Shiga Toxin Receptor Glycolipid Binding

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    Pages 187-195

    Methods for the Purification of Shiga Toxin 1

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    Pages 197-208

    Methods for the Identification of Host Receptors for Shiga Toxin

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    Pages 209-220

    Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport

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    Pages 221-228

    Measuring pH Within the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin

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    Pages 229-241

    Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways

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    Pages 243-262

    Interaction of Shiga Toxin with Endothelial Cells

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