Adhesion Protein Protocols

Editors:

ISBN: 978-0-89603-417-4 (Print) 978-1-59259-258-6 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-9

    Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules

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    Pages 11-20

    Epitope Mapping

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    Pages 21-28

    Sequencing of Antibodies

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    Pages 29-37

    Purification Through Affinity Chromatography and Microsequencing

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    Pages 39-63

    Functional Analysis of Cell Adhesion Molecules

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    Pages 65-76

    Application of Polymerase Chain Reaction for the Discovery of New Adhesion Molecule Family Members

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    Pages 77-84

    Construction and Purification of Adhesion Molecule Immunoglobulin Chimeric Proteins

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    Pages 85-92

    Immunofluorescence of Cultured Cells

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    Pages 93-106

    Detection of Adhesion Molecules by Immunohistochemistry on Human and Murine Tissue Sections

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    Pages 107-117

    In situ Hybridization with RNA Probes

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    Pages 119-124

    Purification of Fibronectin from Human Plasma

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    Pages 125-130

    Adhesion to Matrix Proteins

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    Pages 131-136

    Leukocyte-Endothelial Monolayer Adhesion Assay (Static Conditions)

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    Pages 137-145

    Cell-Cell Adhesive Interactions in an In Vitro Flow Chamber

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    Pages 147-151

    Adhesion of Tumor Cells to Endothelium Under Static Conditions

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    Pages 153-157

    Adhesion of Tumor Cells Under Flow

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    Pages 159-170

    Platelet Adhesion to the Subendothelium Under Flow

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    Pages 171-176

    Leukocyte Transmigration Through Vascular Endothelium

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    Pages 177-182

    Cell Migration into a Wounded Area In Vitro

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    Pages 183-189

    Determination of the Endothelial Barrier Function In Vitro

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