Adipose Tissue Protocols

Editors:

ISBN: 978-0-89603-747-2 (Print) 978-1-59259-231-9 (Online)

Table of contents (26 protocols)

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  1. Front Matter

    Pages i-xiii

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    Pages 1-19

    Choosing an Adipose Tissue Depot for Sampling

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    Pages 21-51

    Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue

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    Pages 53-64

    Confocal Microscopy of Adipocytes

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    Pages 65-75

    Cellularity Measurements

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    Pages 77-82

    Subcellular Fractionation of Adipocytes and 3T3-L1 Cells

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    Pages 83-88

    Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction in Human White Adipose Tissue Biopsies

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    Pages 89-96

    Glycogen Synthase Activity in Adipose Tissue

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    Pages 97-119

    Assays of Lipolytic Enzymes

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    Pages 121-127

    Assays of Lipogenic Enzymes

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    Pages 129-140

    Assays of Adrenergic Receptors

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    Pages 141-146

    Transfection of Adipocytes and Preparation of Nuclear Extracts

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    Pages 147-155

    Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes

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    Pages 157-165

    Assays of Glucose Entry, Glucose Transporter Amount, and Translocation

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    Pages 167-180

    Methods to Study Phosphorylation and Activation of the Hormone-Sensitive Adipocyte Phosphodiesterase Type 3B in Rat Adipocytes

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    Pages 181-192

    Measurements of Glucose Conversion to its Metabolites

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    Pages 193-196

    Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes

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    Pages 197-212

    Culture of Adipose Tissue and Isolated Adipocytes

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    Pages 213-224

    Cultures of Adipose Precursor Cells from Brown Adipose Tissue and of Clonal Brown-Adipocyte-Like Cell Lines

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    Pages 225-237

    Cultures of Adipose Precursor Cells and Cells of Clonal Lines from Animal White Adipose Tissue

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    Pages 239-247

    Cultures of Human Adipose Precursor Cells

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