In Situ Detection of DNA Damage

Methods and Protocols

Editors:

ISBN: 978-0-89603-952-0 (Print) 978-1-59259-179-4 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xvii

  2. Labeling DNA Breaks Using Terminal Transferase (TUNEL Assay)

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-19

      Labeling DNA Damage with Terminal Transferase

    3. No Access

      Book Protocol

      Pages 21-30

      TUNEL Assay

    4. No Access

      Book Protocol

      Pages 31-39

      Electron Microscopic Detection of DNA Damage Labeled by TUNEL

    5. No Access

      Book Protocol

      Pages 41-54

      Quantitative Differentiation of Both Free 3′ OH and 5′ OH DNA Ends Using Terminal Transferase-Based Labeling Combined with Transmission Electron Microscopy

    6. No Access

      Book Protocol

      Pages 55-68

      Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and Quick-Freezing and Deep-Etching Techniques

    7. No Access

      Book Protocol

      Pages 69-75

      In Situ Detection of DNA Strand Breaks in Analysis of Apoptosis by Flow- and Laser-Scanning Cytometry

  3. Labeling DNA Breaks Using DNA Polymerase I or its Klenow Fragment

    1. Front Matter

      Pages 79-79

    2. No Access

      Book Protocol

      Pages 81-108

      DNA Damage Detection Using DNA Polymerase I or its Klenow Fragment

    3. No Access

      Book Protocol

      Pages 109-119

      Labeling DNA Breaks In Situ by Klenow Enzyme

    4. No Access

      Book Protocol

      Pages 121-130

      In Situ Nick Translation at the Electron Microscopic Level

  4. Labeling DNA Breaks Using Ligase

    1. Front Matter

      Pages 131-131

    2. No Access

      Book Protocol

      Pages 133-141

      In Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections

    3. No Access

      Book Protocol

      Pages 143-151

      Detection of Specific Double-Strand DNA Breaks and Apoptosis In Situ Using T4 DNA Ligase

    4. No Access

      Book Protocol

      Pages 153-159

      In Situ Detection of Double-Strand DNA Breaks with Terminal 5′OH Groups

  5. Detection of DNA Breaks in Agarose Trapped Cells: Comet Assay and Related Techniques

    1. Front Matter

      Pages 161-161

    2. No Access

      Book Protocol

      Pages 163-177

      The Comet Assay

    3. No Access

      Book Protocol

      Pages 179-194

      The Comet Assay

    4. No Access

      Book Protocol

      Pages 195-201

      Ultrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL Assays

    5. No Access

      Book Protocol

      Pages 203-216

      Application of FISH to Detect DNA Damage

  6. Detection of Modified Bases and AP Sites in DNA

    1. Front Matter

      Pages 217-217

    2. No Access

      Book Protocol

      Pages 219-234

      Simultaneous In Situ Detection of DNA Fragmentation and RNA/DNA Oxidative Damage Using TUNEL Assay and Immunohistochemical Labeling for 8-Hydroxy-2′-Deoxyguanosine (8-OHdG)

    3. No Access

      Book Protocol

      Pages 235-244

      The In Situ Detection of Apurinic/Apyrimidinic Sites and DNA Breaks Bearing Extension Blocking Termini

  7. Indirect and General Markers of DNA Damage

    1. Front Matter

      Pages 245-245

    2. No Access

      Book Protocol

      Pages 247-255

      Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage

    3. No Access

      Book Protocol

      Pages 257-277

      Ultrasound Imaging of Apoptosis

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