Book 2002

The Protein Protocols Handbook

Editors:

ISBN: 978-0-89603-940-7 (Print) 978-1-59259-169-5 (Online)

Table of contents (164 protocols)

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  1. Front Matter

    Pages i-xxiv

  2. Quantitation of Proteins

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-6

      Protein Determination by UV Absorption

    3. No Access

      Protocol

      Pages 7-9

      The Lowry Method for Protein Quantitation

    4. No Access

      Protocol

      Pages 11-14

      The Bicinchoninic Acid (BCA) Assay for Protein Quantitation

    5. No Access

      Protocol

      Pages 15-21

      The Bradford Method for Protein Quantitation

    6. No Access

      Protocol

      Pages 23-30

      Ultrafast Protein Determinations Using Microwave Enhancement

    7. No Access

      Protocol

      Pages 31-40

      The Nitric Acid Method for Protein Estimation in Biological Samples

    8. No Access

      Protocol

      Pages 41-44

      Quantitation of Tryptophan in Proteins

    9. No Access

      Protocol

      Pages 45-50

      Flow Cytometric Quantitation of Cellular Proteins

    10. No Access

      Protocol

      Pages 51-54

      Kinetic Silver Staining of Proteins

  3. Electrophoresis of Proteins and Peptides and Detection in Gels

    1. Front Matter

      Pages 55-55

    2. No Access

      Protocol

      Pages 57-60

      Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins

    3. No Access

      Protocol

      Pages 61-67

      SDS Polyacrylamide Gel Electrophoresis of Proteins

    4. No Access

      Protocol

      Pages 69-72

      Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins

    5. No Access

      Protocol

      Pages 73-79

      SDS-Polyacrylamide Gel Electrophoresis of Peptides

    6. No Access

      Protocol

      Pages 81-86

      Identification of Nucleic Acid Binding Proteins Using Nondenaturing Sodium Decyl Sulfate Polyacrylamide Gel Electrophoresis (SDecS-PAGE)

    7. No Access

      Protocol

      Pages 87-101

      Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins

    8. No Access

      Protocol

      Pages 103-111

      Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins

    9. No Access

      Protocol

      Pages 113-123

      Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones

    10. No Access

      Protocol

      Pages 125-129

      Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels

    11. No Access

      Protocol

      Pages 131-140

      Protein Solubility in Two-Dimensional Electrophoresis

    12. No Access

      Protocol

      Pages 141-157

      Preparation of Protein Samples from Mouse and Human Tissues for 2-D Electrophoresis

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