Meningococcal Vaccines

Methods and Protocols

Editors:

ISBN: 978-0-89603-801-1 (Print) 978-1-59259-148-0 (Online)

Table of contents (28 protocols)

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  1. Front Matter

    Pages i-xvii

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    Pages 1-22

    Meningococcal Vaccines and Vaccine Developments

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    Pages 23-39

    Immune Response and Host-Pathogen Interactions

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    Pages 41-47

    Purification of Capsular Polysaccharide

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    Pages 49-54

    Protein-Polysaccharide Conjugation

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    Pages 55-60

    N-Propionylation

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    Pages 61-79

    Outer Membrane Protein Purification

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    Pages 81-107

    Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease

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    Pages 109-120

    Methods for Manipulation of Transferrin-Binding Proteins

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    Pages 121-128

    Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. meningitidis Outer Membrane Proteins

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    Pages 129-143

    Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics

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    Pages 145-154

    Construction of porA Mutants

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    Pages 155-165

    Construction of LPS Mutants

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    Pages 167-180

    Recombinant Proteins in Vaccine Development

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    Pages 181-197

    Identification of Peptides that Mimic N. meningitidis LOS Epitopes Via the Use of Combinatorial Phage-Display Libraries

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    Pages 199-221

    Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity

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    Pages 223-239

    Early Experiences with Nasal Vaccines against Meningococcal Disease

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    Pages 241-254

    Animal Models for Meningococcal Disease

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    Pages 255-273

    Determination of Antibody Responses to Meningococcal Antigens by ELISA

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    Pages 275-288

    Immunoblot Analysis of Sera from Patients and Vaccinees

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    Pages 289-304

    Serogroup B and C Serum Bactericidal Assays

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