Developmental Biology Protocols

Editors:

ISBN: 978-0-89603-854-7 (Print) 978-1-59259-066-7 (Online)

Table of contents (45 protocols)

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  1. Front Matter

    Pages i-xxv

  2. Introduction

    1. Front Matter

      Pages 1-1

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      Pages 3-5

      Developmental Biology Protocols

  3. Manipulation Of Developmental Gene Expression and Function

    1. Front Matter

      Pages 7-7

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      Pages 9-14

      Ectopic Expression in Drosophila

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      Pages 15-22

      Clonal Analysis in the Examination of Gene Function in Drosophila

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      Pages 23-36

      Application of Antisense Oligodeoxynucleotides in Developing Chick Embryos

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      Pages 37-42

      Application of Functional Blocking Antibodies

  4. Analysis of Gene Expression

    1. Front Matter

      Pages 43-43

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      Pages 45-50

      Gene Expression Analyzed by Ribonuclease Protection Assay

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      Pages 51-58

      Relative Reverse Transcription-Polymerase Chain Reaction

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      Pages 59-73

      Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Multispecific Internal Control

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      Pages 75-86

      In Situ PCR Detection of HIV Expression in the Human Placenta

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      Pages 87-96

      Gene Expression Analysis by In Situ Hybridization

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      Pages 97-115

      Radio-Isotopic In Situ Hybridization on Tissue Sections

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      Pages 117-124

      mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry

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      Pages 125-137

      Whole Mount In Situ Hybridization to Study Gene Expression During Mouse Development

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      Pages 139-148

      Multicolor Whole-Mount In Situ Hybridization

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      Pages 149-152

      Methods for Double Detection of Gene Expression

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      Pages 153-157

      Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins

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      Pages 159-166

      Monoclonal Antibodies in the Analysis of Embryonic Development

  5. Models of Morphogenesis and Development

    1. Front Matter

      Pages 167-167

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      Pages 169-178

      Mesoderm Induction in Xenopus

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      Pages 179-183

      Amphibian Organizer Activity

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      Pages 185-199

      Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery

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