Mass Spectrometry of Proteins and Peptides

Mass Spectrometry of Proteins and Peptides

Editors:

ISBN: 978-0-89603-609-3 (Print) 978-1-59259-045-2 (Online)

Table of contents (27 protocols)

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  1. Front Matter

    Pages i-xiv

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    Pages 1-16

    De Novo Peptide Sequencing by Nanoelectrospray Tandem Mass Spectrometry Using Triple Quadrupole and Quadrupole/Time-of-Flight Instruments

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    Pages 17-26

    Direct Analysis of Proteins in Mixtures

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    Pages 27-39

    Characterization of a Mutant Recombinant S100 Protein Using Electrospray Ionization Mass Spectrometry

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    Pages 41-61

    Searching Sequence Databases via De Novo Peptide Sequencing by Tandem Mass Spectrometry

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    Pages 63-94

    Signature Peptides From Analytical Chemistry to Functional Genomics

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    Pages 95-112

    Investigating the Higher Order Structure of Proteins

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    Pages 113-131

    Probing Protein Surface Topology by Chemical Surface Labeling, Crosslinking, and Mass Spectrometry

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    Pages 133-146

    Secondary Structure of Peptide Ions in the Gas Phase Evaluated by MIKE Spectrometry

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    Pages 147-165

    Preparation and Mass Spectrometric Analysis of S-Nitrosohemoglobin

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    Pages 167-184

    Multiple and Subsequent MALDI-MS on-target Chemical Reactions for the Characterization of Disulfide Bonds and Primary Structures of Proteins

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    Pages 185-201

    Epitope Mapping by a Combination of Epitope Excision and MALDI-MS

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    Pages 203-222

    Identification of Active Site Residues in Glycosidases by Use of Tandem Mass Spectrometry

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    Pages 223-238

    Probing Protein-Protein Interactions with Mass Spectrometry

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    Pages 239-249

    Studies of Noncovalent Complexes in an Electrospray lonization/ Time-of-Flight Mass Spectrometer

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    Pages 251-272

    Kinetic Analysis of Enzymatic and Nonenzymatic Degradation of Peptides by MALDI-TOFMS

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    Pages 273-292

    Characterization of Protein Glycosylation by MALDI-TOFMS

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    Pages 293-315

    Positive and Negative Labeling of Human Proinsulin, Insulin, and C-Peptide with Stable Isotopes

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    Pages 317-335

    Identification of Snake Species by Toxin Mass Fingerprinting of Their Venoms

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    Pages 337-354

    Mass Spectrometric Characterization of the β-Subunit of Human Chorionic Gonadotropin

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    Pages 355-367

    Analysis of Gluten in Foods by MALDI-TOFMS

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