Aqueous Two-Phase Systems: Methods and Protocols

Methods and Protocols

Editors:

ISBN: 978-0-89603-541-6 (Print) 978-1-59259-028-5 (Online)

Table of contents (38 protocols)

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  1. Front Matter

    Pages i-xiii

  2. Preparation and Characterization of Aqueous Two-Phase Systems

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      Pages 1-10

      Aqueous Two-Phase Systems

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      Pages 11-21

      The Phase Diagram

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      Pages 23-33

      Preparation of Aqueous Two-Phase Systems

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      Pages 35-45

      Measurement of Some Physical Properties of Aqueous Two-Phase Systems

  3. Partitioning Techniques

    1. Front Matter

      Pages 11-45

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      Pages 47-54

      Single-Step Partitioning in Aqueous Two-Phase Systems

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      Pages 55-64

      Partition by Countercurrent Distribution (CCD)

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      Pages 65-75

      Liquid-Liquid Partition Chromatography (LLPC)

  4. Partitioning of Soluble Molecules and Particulates

    1. Front Matter

      Pages 46-75

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      Pages 77-94

      Metal Ion Separations in Aqueous Biphasic Systems and with ABEC™ Resins

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      Pages 95-105

      Partition of Amino Acids and Peptides in Aqueous Two-Phase Systems

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      Pages 107-118

      Predicting Partition Coefficients of Small Solutes Based on Hydrophobicity

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      Pages 119-142

      Eukaryotic Cell Partition

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      Pages 143-158

      Concentration and Purification of Viruses

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      Pages 159-166

      Isolation of Plant Plasma Membranes and Production of Inside-Out Vesicles

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      Pages 167-175

      Isolation of Inside-Out Thylakoid Vesicles

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      Pages 177-184

      Two-Phase Partitioning as a Method for Isolation of Tight Plasma Membrane Vesicles from Saccharomyces cerevisiae and from Chlamydomonas reinhardtii

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      Pages 185-192

      Purification of Cyanobacterial Thylakoid, Plasma, and Outer Membranes by Two-Phase Partitioning

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      Pages 193-200

      Purification of Plasma Membranes by Affinity Partitioning

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      Pages 201-208

      Studying the Influence of Salts on Partitioning of Proteins

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      Pages 209-218

      Partitioning of Chemically Modified Proteins

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      Pages 219-228

      Detection and Analysis of Interactions by Two-Phase Partition

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