Adenovirus Methods and Protocols

Editors:

ISBN: 978-0-89603-551-5 (Print) 978-1-59259-603-4 (Online)

Table of contents (25 protocols)

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  1. Front Matter

    Pages i-xiii

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    Pages 1-9

    Preparation and Titration of CsCl-Banded Adenovirus Stock

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    Pages 11-24

    Construction of Mutations in the Adenovirus Early Region 3 (E3) Transcription Units

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    Pages 25-32

    Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants

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    Pages 33-46

    Manipulation of Early Region 4

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    Pages 47-59

    Adenovirus DNA Packaging

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    Pages 61-83

    Methods for Creating and Analyzing Adenovirus Vectors that Express Proteins that Act on the Viral Genome

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    Pages 85-103

    Construction of Mouse Adenovirus Type 1 Mutants

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    Pages 105-111

    Generation of Adenovirus-Specific Cytotoxic T Lymphocytes

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    Pages 113-120

    Measurement of Macrophage-Mediated Cytolysis of Adenovirus-Infected Cells

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    Pages 121-128

    Measurement of Tumor Necrosis Factor (TNF) Lysis of Adenovirus-Infected Cells

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    Pages 129-141

    Role of Natural Killer (NK) Cells in Immunity to Adenoviruses

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    Pages 143-149

    Determination of the Transforming Activities of Adenovirus Oncogenes

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    Pages 151-156

    Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members

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    Pages 157-168

    In Vitro Transcription

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    Pages 169-193

    Cell Microinjection

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    Pages 195-202

    Immunoprecipitation of E1 A-Containing Protein Complexes

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    Pages 203-216

    Preparation of Splicing-Competent Nuclear Extracts from Adenovirus-lnfected Cells

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    Pages 217-230

    Adenovirus Entry into Cells

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    Pages 231-240

    Simultaneous Detection of RNA and Proteins in Adenovirus-Infected Cells by Fluorescence In Situ Hybridization (FISH) and lmmunostaining

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    Pages 241-257

    Characterization of the Adenovirus Fiber Protein

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