Animal Cell Biotechnology

Methods and Protocols

Editors:

ISBN: 978-0-89603-547-8 (Print) 978-1-59259-486-3 (Online)

Table of contents (23 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Enabling Technologies

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-9

      Setting Up a New Cell Culture Laboratory

    3. No Access

      Book Protocol

      Pages 11-22

      Adaptation of Mammalian Cells to Growth in Serum-Free Media

    4. No Access

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      Pages 23-36

      Viral Evaluation of Animal Cell Lines Used in Biotechnology

  3. Molecular Methods

    1. Front Matter

      Pages 37-37

    2. No Access

      Book Protocol

      Pages 39-48

      Optimizing Gene Expression in Mammalian Cells

    3. No Access

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      Pages 49-59

      Cytogenetic Characterization of Recombinant Cells

    4. No Access

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      Pages 61-72

      Immortalization Strategies for Mammalian Cells

    5. No Access

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      Pages 73-98

      Mouse Monoclonal Antibodies

    6. Book Protocol

      Pages 99-115

      Cell Bank Preparation and Characterization

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      Pages 117-127

      DNA Fingerprinting and Characterization of Animal Cells

  4. Cell Evaluation Protocols

    1. Front Matter

      Pages 129-129

    2. No Access

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      Pages 131-144

      Cell Counting and Viability Measurements

    3. No Access

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      Pages 145-153

      Monitoring Animal Cell Growth and Productivity by Flow Cytometry

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      Pages 155-164

      Measurement of Cell Death in Culture

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      Pages 165-175

      Nuclear Magnetic Resonance Methods of Monitoring Cell Metabolism

  5. Specialist Techniques

    1. Front Matter

      Pages 177-177

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      Pages 179-185

      Culturing Animal Cells in Fluidized Bed Reactors

    3. No Access

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      Pages 187-200

      GPI-Anchored Fusion Proteins

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      Pages 201-209

      Harvesting GPI-Anchored Proteins From CHO Cells

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      Pages 211-227

      Hematopoietic Cells for Cellular and Gene Therapy

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      Pages 229-238

      Hematopoietic Cells for Cellular and Gene Therapy

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      Pages 239-252

      Cytotoxicity Testing Using Cell Lines

  6. Product Evaluation Protocols

    1. Front Matter

      Pages 253-253

    2. No Access

      Book Protocol

      Pages 255-261

      Measuring the Folding Dynamics of Recombinant Proteins Secreted from Mammalian Cells

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