PCR in Bioanalysis

Editors:

ISBN: 978-0-89603-497-6 (Print) 978-1-59259-575-4 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-29

    Application of Nucleic Acid Amplification in Clinical Microbiology

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    Pages 31-37

    Detection of Rodent Parvoviruses by PCR

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    Pages 39-47

    The SURF Technique

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    Pages 49-54

    Prenatal and Presymptomatic Diagnosis of Marfan Syndrome Using Fluorescence PCR and an Automated Sequencer

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    Pages 55-66

    Measurement of TNF and iNOS mRNA Using cDNA-Equalized Reverse Transcriptase PCR

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    Pages 67-79

    PCR Diagnosis of the Bovine Immunodeficiency-Like Virus

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    Pages 81-88

    PCR Analysis for the Identification of Porcine Reproductive and Respiratory Syndrome Virus in Boar Semen

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    Pages 89-100

    Quantitative and Discriminative Detection of Individual HIV-1 mRNA Subspecies by an RNase Mapping Assay

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    Pages 101-114

    Detection of the Heat-Labile Toxin Coding Gene (LT-Gene) of Enterotoxigenic Escherichia coli

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    Pages 115-125

    Motif-Dependent Polymerase Chain Reaction (PCR)

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    Pages 127-144

    In Vitro Transcription/Translation Analysis for the Identification of Translation-Terminating Mutations

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    Pages 145-152

    A Single-Tube Nested RT-PCR for the Detection of Ross River Virus

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    Pages 153-164

    Application of PCR to Transgenic Plants

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    Pages 165-171

    Detection of Hepatitis C Virus RNA by Semiquantitative Reverse-Transcription PCR

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    Pages 173-181

    Detection of Legionella Species in Bronchial Fluid by PCR

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    Pages 183-193

    Quantitative Measurement of mRNA Expression by Competitive RT-PCR

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    Pages 195-202

    PCR Detection of Toxoplasma gondii in Human Fetal Tissues

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    Pages 203-214

    Rapid Diagnosis of Pulmonary Tuberculosis Using Roche AMPLICOR™Mycobacterium tuberculosis PCR Test

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    Pages 215-225

    The Use of Flow Cytometry and RT-PCR in the Detection of Circulating PSA-Positive Cells in Prostate Cancer

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    Pages 227-237

    Detection of Chlamydia trachomatis and Trichomonas vaginalis in the Vaginal Introitus, Posterior Vagina, and Endocervix by Polymerase Chain Reaction

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