Book Volume 108 1998

Free Radical and Antioxidant Protocols

Editors:

ISBN: 978-0-89603-472-3 (Print) 978-1-59259-254-8 (Online)

Table of contents (40 protocols)

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  1. Front Matter

    Pages i-xvii

  2. Techniques for the Measurement of Oxidative Stress

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-14

      Oxygen Consumption Methods

    3. No Access

      Protocol

      Pages 15-25

      Spin Trapping and Electron Paramagnetic Resonance Spectroscopy

    4. No Access

      Protocol

      Pages 27-35

      In Vivo Detection of Free Radicals in Real Time by Low-Frequency Electron Paramagnetic Resonance Spectroscopy

    5. No Access

      Protocol

      Pages 37-55

      Single Photon Counting

    6. No Access

      Protocol

      Pages 57-62

      Measurement of Reactive Oxygen Species in Whole Blood and Mononuclear Cells Using Chemiluminescence

    7. No Access

      Protocol

      Pages 63-70

      Assay of Phospholipid Hydroperoxides by Chemiluminescence-Based High-Performance Liquid Chromatography

    8. No Access

      Protocol

      Pages 71-87

      Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid

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      Protocol

      Pages 89-100

      Aromatic Hydroxylation

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      Protocol

      Pages 101-106

      Simple Assay for the Level of Total Lipid Peroxides in Serum or Plasma

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      Protocol

      Pages 107-110

      Simple Procedure for Specific Assay of Lipid Hydroperoxides in Serum or Plasma

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      Protocol

      Pages 111-118

      Antibodies Against Malondialdehyde-Modified Proteins

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      Protocol

      Pages 119-130

      Oxidized LDL and Lp(a)

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      Protocol

      Pages 131-137

      Oxidized and Unoxidized Fatty Acyl Esters

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      Protocol

      Pages 139-145

      Synthesis of Lipid and Cholesterol Hydroperoxide Standards

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      Protocol

      Pages 147-155

      Separation of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids By High-Pressure Liquid Chromatography

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      Protocol

      Pages 157-164

      Products of Creatinine with Hydroxyl Radical as a Useful Marker of Oxidative Stress In Vivo

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      Protocol

      Pages 165-180

      Quantitative Analysis of Peptide and Protein Changes in Ischemic Hippocampal Tissue by HPLC

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      Protocol

      Pages 181-190

      Electrochemical Detection of 8-Hydroxy-2-Deoxyguanosine Levels in Cellular DNA

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      Protocol

      Pages 191-198

      Nitric Oxide Synthase

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      Protocol

      Pages 199-209

      Quantitation of Heme Oxygenase (HO-1) Copies in Human Tissues by Competitive RT/PCR

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