Microarray Technology and Its Applications

1. Front Matter

   Pages I-XXII

   PDF

2. General Microarray Technologies

   1. Array Formats

      • Ralph R. Martel, Matthew P. Rounseville, Ihab W. Botros, Bruce E. Seligmann

      Pages 3-22

   2. Biomolecules and Cells on Surfaces — Fundamental Concepts

      • Kristi L. Hanson, Luisa Filipponi, Dan V. Nicolau

      Pages 23-44

   3. Surfaces and Substrates

      • Alvaro Carrillo, Kunal V. Gujraty, Ravi S. Kane

      Pages 45-61

   4. Reagent Jetting Based Deposition Technologies for Array Construction

      • Mitchel J. Doktycz

      Pages 63-72

   5. Manufacturing of 2-D Arrays by Pin-printing Technologies

      • Uwe R. Müller, Roeland Papen

      Pages 73-88

   6. Nanoarrays

      • Dan V. Nicolau, Linnette Demers, David S. Ginger

      Pages 89-118

   7. The Use of Microfluidic Techniques in Microarray Applications

      • Piotr Grodzinski, Robin H. Liu, Ralf Lenigk, Yingjie Liu

      Pages 119-145

   8. Labels and Detection Methods

      • James J. Storhoff, Sudhakar S. Marla, Viswanadham Garimella, Chad A. Mirkin

      Pages 147-179

   9. Marker-free Detection on Microarrays

      • Matthias Vaupel, Andreas Eing, Karl-Otto Greulich, Jan Roegener, Peter Schellenberg, Hans Martin. Striebel et al.

      Pages 181-207

3. DNA Microarrays

   1. Analysis of DNA Sequence Variation in the Microarray Format

      • Ulrika Liljedahl, Mona Fredriksson, Ann-Christine Syvänen

      Pages 211-227

   2. High Sensitivity Expression Profiling

      • Ramesh Ramakrishnan, Paul Bao, Uwe R. Müller

      Pages 229-250

   3. Applications of Matrix-CGH (Array-CGH) for Genomic Research and Clinical Diagnostics

      • Carsten Schwaenena, Michelle Nesslinga, Bernhard Radlwimmera, Swen Wessendorf, Peter Lichtera

      Pages 251-263

   4. Analysis of Gene Regulatory Circuits

      • Zirong Li

      Pages 265-276

4. Protein Microarrays

   1. Protein, Antibody and Small Molecule Microarrays

      • Hendrik Weiner, Jörn Glökler, Claus Hultschig, Konrad Büssow, Gerald Walter

      Pages 279-295

   2. Photoaptamer Arrays for Proteomics Applications

      • Drew Smith, Chad Greef

      Pages 297-308

   3. Biological Membrane Microarrays

      • Ye Fang, Anthony G. Frutos, Yulong Hong, Joydeep Lahiri

      Pages 309-320

5. Cell & Tissue Microarrays

   1. Use of Reporter Systems for Reverse Transfection Cell Arrays

      • Brian L. Webb

      Pages 323-333

   2. Whole Cell Microarrays

      • Ravi Kapur

      Pages 335-343

   3. Tissue Microarrays for Miniaturized High-Throughput Molecular Profiling of Tumors

      • Ronald Simon, Martina Mirlacher, Guido Sauter

      Pages 345-360

Ithasbeenstatedthatourknowledgedoublesevery20years,butthatmaybe an understatement when considering the Life Sciences. A series of discoveries and inventions have propelled our knowledge from the recognition that DNA isthegeneticmaterialtoabasicmolecularunderstandingofourselvesandthe living world around us in less than 50 years. Crucial to this rapid progress was thediscoveryofthedouble-helicalstructureofDNA,whichlaidthefoundation forallhybridizationbasedtechnologies. Thediscoveriesofrestrictionenzymes, ligases, polymerases, combined with key innovations in DNA synthesis and sequencing ushered in the era of biotechnologyas a new science with profound sociological and economic implications that are likely to have a dominating in?uence on the development of our society during this century. Given the process by which science builds on prior knowledge, it is perhaps unfair to single out a few inventions and credit them with having contributed most to thisavalancheofknowledge. Yet,therearesurelysomethatwillberecognized as having had a more profound impact than others, not just in the furthering of our scienti?c knowledge, but by leveraging commercial applications that provide a tangible return to our society. The now famous Polymerase Chain Reaction, or PCR, is surely one of those, as it has uniquely catalyzed molecular biology during the past 20 years, and continues to have a signi?cant impact on all areas that involve nucleic acids, ranging from molecular pathology to forensics. Ten years ago micro- ray technology emerged as a new and powerful tool to study nucleic acid - quences in a highly multiplexed manner, and has since found equally exciting and useful applications in the study of proteins, metabolites, toxins, viruses, whole cells and even tissues.

• DNA
• bioinformatics
• cell
• deposition
• development
• diagnostics
• genes
• genomics
• instrumentation
• membrane
• microarray
• protein
• surface
• tissue
• translation

• V.P., Applied Science Nanosphere, Inc., Northbrook, USA

  Uwe R. Müller

• Swinburne University of Technology, Hawthorn, Australia

  Dan V. Nicolau

Policies and ethics