Authors:
- Nominated by Chinese Academy of Sciences as an outstanding PhD thesis
- Describes the first example of online multidimensional chromatography separation using capillary monolithic columns
- Introduces the combination of online stable isotope labeling and multidimensional chromatography separation
- Outlines the proteome and phosphoproteome expression profiles of clinical hepatocellular carcinoma tissues and hippocampi of mice with Alzheimer’s disease
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Theses (Springer Theses)
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Table of contents (4 chapters)
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Front Matter
About this book
In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.
Keywords
- Isotope Dimethylation Labeling
- Mass Spectrometry
- Multidimensional Chromatography Separation
- Polymer-Based Hydrophobic Monolithic Column
- Protein Phosphorylation
- Proteome Analysis
- Quantitative Proteomics
- Separation of Complex Peptide Mixtures
- Stable Isotope Labeling
- Strong Cation-Exchange Monolithic Column
- chromatography
- Proteomics
Authors and Affiliations
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Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, People's Republic of China
Fangjun Wang
Bibliographic Information
Book Title: Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis
Authors: Fangjun Wang
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-3-642-42008-5
Publisher: Springer Berlin, Heidelberg
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer-Verlag Berlin Heidelberg 2014
Hardcover ISBN: 978-3-642-42007-8Published: 17 December 2013
Softcover ISBN: 978-3-662-52432-9Published: 27 August 2016
eBook ISBN: 978-3-642-42008-5Published: 02 December 2013
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XIII, 94
Number of Illustrations: 19 b/w illustrations, 23 illustrations in colour
Topics: Chromatography, Proteomics, Mass Spectrometry