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Cell-Free Protein Synthesis

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Kirill Alexandrov

    1. Institute for Molecular Bioscience (IMB), The University of Queensland, St. Lucia, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Wayne A. Johnston

    1. Institute for Molecular Bioscience (IMB), The University of Queensland, St. Lucia, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1118)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae

    • Wayne A. Johnston, Kirill Alexandrov
    Pages 1-15

  3. Bioinformatics Analysis and Optimization of Cell-Free Protein Synthesis

    • Alexander A. Tokmakov, Atsushi Kurotani, Mikako Shirouzu, Yasuo Fukami, Shigeyuki Yokoyama
    Pages 17-33

  4. A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression

    • Andrew Kralicek
    Pages 35-54

  5. One-Pot, Microscale Cell-Free Enzyme Expression and Screening

    • Aarthi Chandrasekaran, Anup K. Singh
    Pages 55-69

  6. Cell-Free Translation of Biofuel Enzymes

    • Taichi E. Takasuka, Johnnie A. Walker, Lai F. Bergeman, Kirk A. Vander Meulen, Shin-ichi Makino, Nathaniel L. Elsen et al.
    Pages 71-95

  7. Cloning-Independent Expression and Screening of Enzymes Using Cell-Free Protein Synthesis Systems

    • Yong-Chan Kwon, Jae-Kwang Song, Dong-Myung Kim
    Pages 97-108

  8. High-Level Cell-Free Production of Membrane Proteins with Nanodiscs

    • Christian Roos, Lei Kai, Stefan Haberstock, Davide Proverbio, Umesh Ghoshdastider, Yi Ma et al.
    Pages 109-130

  9. Cell-Free Protein-Based Enzyme Discovery and Protein–Ligand Interaction Study

    • Sabrina Guillemer, Cécile Persillon, Jean-Michel Masson, Gilles Ravot
    Pages 131-147

  10. Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis

    • Tominari Kobayashi, Kodai Machida, Hiroaki Imataka
    Pages 149-156

  11. Cell-Free Protein Synthesis in Microfluidic 96-Well Plates

    • Kirsten Jackson, Ruba Khnouf, Z. Hugh Fan
    Pages 157-168

  12. Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis

    • Kiyoshi Ozawa, Ruhu Qi
    Pages 169-187

  13. Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis

    • Kiyoshi Ozawa, Choy Theng Loh
    Pages 189-203

  14. In Vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate

    • Kong-Nan Zhao
    Pages 205-218

  15. An Optimized Yeast Cell-Free Lysate System for In Vitro Translation of Human Virus mRNA

    • Xiao Wang, Liang Zhao, Kong-Nan Zhao
    Pages 219-230

  16. In Vitro Translation-Based Protein Kinase Substrate Identification

    • Szilvia K. Nagy, Tamás Mészáros
    Pages 231-243

  17. Preparation of Protein Arrays Using Cell-Free Protein Expression

    • Elizabeth A. Cook, Mingyue He
    Pages 245-255

  18. Posttranscriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System

    • Motoaki Wakiyama, Shigeyuki Yokoyama
    Pages 257-266

  19. Cell-Free Membrane Protein Expression

    • Tomomi Kimura-Soyema, Mikako Shirouzu, Shigeyuki Yokoyama
    Pages 267-273

  20. The PURE System for Protein Production

    • Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, Takuya Ueda
    Pages 275-284
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Keywords

About this book

Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies.

Editors and Affiliations

  • Institute for Molecular Bioscience (IMB), The University of Queensland, St. Lucia, Australia

    Kirill Alexandrov, Wayne A. Johnston

Bibliographic Information

  • Book Title: Cell-Free Protein Synthesis

  • Book Subtitle: Methods and Protocols

  • Editors: Kirill Alexandrov, Wayne A. Johnston

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-782-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-781-5Published: 09 January 2014

  • Softcover ISBN: 978-1-4939-6254-9Published: 27 August 2016

  • eBook ISBN: 978-1-62703-782-2Published: 06 January 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 313

  • Number of Illustrations: 35 b/w illustrations, 31 illustrations in colour

  • Topics: Protein Science

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